Role of OmpA in the multidrug resistance phenotype of Acinetobacter baumannii

doi:10.1128/AAC.02101-13

. 2014;58(3):1806-8.

doi: 10.1128/AAC.02101-13. Epub 2013 Dec 30.

Role of OmpA in the multidrug resistance phenotype of Acinetobacter baumannii

Younes Smani¹, Anna Fàbrega, Ignasi Roca, Viviana Sánchez-Encinales, Jordi Vila, Jerónimo Pachón

Affiliations

Affiliation

¹ Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Seville, Spain.

PMID: 24379205
PMCID: PMC3957889
DOI: 10.1128/AAC.02101-13

Role of OmpA in the multidrug resistance phenotype of Acinetobacter baumannii

Younes Smani et al. Antimicrob Agents Chemother. 2014.

. 2014;58(3):1806-8.

doi: 10.1128/AAC.02101-13. Epub 2013 Dec 30.

Authors

Younes Smani¹, Anna Fàbrega, Ignasi Roca, Viviana Sánchez-Encinales, Jordi Vila, Jerónimo Pachón

Affiliation

¹ Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Seville, Spain.

PMID: 24379205
PMCID: PMC3957889
DOI: 10.1128/AAC.02101-13

Abstract

Acinetobacter baumannii has emerged as a nosocomial pathogen with an increased prevalence of multidrug-resistant strains. The role of the outer membrane protein A (OmpA) in antimicrobial resistance remains poorly understood. In this report, disruption of the ompA gene led to decreased MICs of chloramphenicol, aztreonam, and nalidixic acid. We have characterized, for the first time, the contribution of OmpA in the antimicrobial resistance phenotype of A. baumannii.

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Figures

**FIG 1**
OMP profile and immunodetection of OmpA of A. baumannii strains. OMPs were extracted from the outer membrane of ATCC 17978 wt, JPAB01, and JPAB01+pJPAB01 strains and stained with Simply Blue SafeStain (SDS-PAGE) or electrotransferred onto a nitrocellulose membrane (Western blot). OMPs were probed with mouse anti-OmpA and horseradish peroxidase-conjugated goat anti-mouse IgG. Molecular mass standards (kDa) are shown on the right.

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References

1. Dijkshoorn L, Nemec A, Seifert H. 2007. An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nat. Rev. Microbiol. 5:939–951. 10.1038/nrmicro1789 - DOI - PubMed
1. Smith SG, Mahon V, Lambert MA, Fagan RP. 2007. A molecular Swiss army knife: OmpA structure, function and expression. FEMS Microbiol. Lett. 273:1–11. 10.1111/j.1574-6968.2007.00778.x - DOI - PubMed
1. Choi CH, Hyun SH, Lee JY, Lee JS, Lee YS, Kim SA, Chae JP, Yoo SM, Lee JC. 2008. Acinetobacter baumannii outer membrane protein A targets the nucleus and induces cytotoxicity. Cell. Microbiol. 10:309–319. 10.1111/j.1462-5822.2007.01041.x - DOI - PubMed
1. Choi CH, Lee EY, Lee Y, Park T, Kim H, Hyun S, Kim S, Lee S, Lee J. 2005. Outer membrane protein 38 of Acinetobacter baumannii localizes to the mitochondria and induces apoptosis of epithelial cells. Cell. Microbiol. 7:1127–1138. 10.1111/j.1462-5822.2005.00538.x - DOI - PubMed
1. Gaddy JA, Tomaras AP, Actis LA. 2009. The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells. Infect. Immun. 77:3150–3160. 10.1128/IAI.00096-09 - DOI - PMC - PubMed

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[1] Dijkshoorn L, Nemec A, Seifert H. 2007. An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nat. Rev. Microbiol. 5:939–951. 10.1038/nrmicro1789 - DOI - PubMed

[2] Dijkshoorn L, Nemec A, Seifert H. 2007. An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nat. Rev. Microbiol. 5:939–951. 10.1038/nrmicro1789 - DOI - PubMed

[3] Smith SG, Mahon V, Lambert MA, Fagan RP. 2007. A molecular Swiss army knife: OmpA structure, function and expression. FEMS Microbiol. Lett. 273:1–11. 10.1111/j.1574-6968.2007.00778.x - DOI - PubMed

[4] Smith SG, Mahon V, Lambert MA, Fagan RP. 2007. A molecular Swiss army knife: OmpA structure, function and expression. FEMS Microbiol. Lett. 273:1–11. 10.1111/j.1574-6968.2007.00778.x - DOI - PubMed

[5] Choi CH, Hyun SH, Lee JY, Lee JS, Lee YS, Kim SA, Chae JP, Yoo SM, Lee JC. 2008. Acinetobacter baumannii outer membrane protein A targets the nucleus and induces cytotoxicity. Cell. Microbiol. 10:309–319. 10.1111/j.1462-5822.2007.01041.x - DOI - PubMed

[6] Choi CH, Hyun SH, Lee JY, Lee JS, Lee YS, Kim SA, Chae JP, Yoo SM, Lee JC. 2008. Acinetobacter baumannii outer membrane protein A targets the nucleus and induces cytotoxicity. Cell. Microbiol. 10:309–319. 10.1111/j.1462-5822.2007.01041.x - DOI - PubMed

[7] Choi CH, Lee EY, Lee Y, Park T, Kim H, Hyun S, Kim S, Lee S, Lee J. 2005. Outer membrane protein 38 of Acinetobacter baumannii localizes to the mitochondria and induces apoptosis of epithelial cells. Cell. Microbiol. 7:1127–1138. 10.1111/j.1462-5822.2005.00538.x - DOI - PubMed

[8] Choi CH, Lee EY, Lee Y, Park T, Kim H, Hyun S, Kim S, Lee S, Lee J. 2005. Outer membrane protein 38 of Acinetobacter baumannii localizes to the mitochondria and induces apoptosis of epithelial cells. Cell. Microbiol. 7:1127–1138. 10.1111/j.1462-5822.2005.00538.x - DOI - PubMed

[9] Gaddy JA, Tomaras AP, Actis LA. 2009. The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells. Infect. Immun. 77:3150–3160. 10.1128/IAI.00096-09 - DOI - PMC - PubMed

[10] Gaddy JA, Tomaras AP, Actis LA. 2009. The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells. Infect. Immun. 77:3150–3160. 10.1128/IAI.00096-09 - DOI - PMC - PubMed

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Role of OmpA in the multidrug resistance phenotype of Acinetobacter baumannii

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Role of OmpA in the multidrug resistance phenotype of Acinetobacter baumannii

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