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. 1995 Dec;15(3-4):301-4.
doi: 10.1007/BF00193741.

Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts

M Salmenkallio-Marttila  1 , K Aspegren, S Akerman, U Kurtén, L Mannonen, A Ritala, T H Teeri, V Kauppinen
Affiliations

Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts

M Salmenkallio-Marttila et al. Plant Cell Rep. 1995 Dec.

Abstract

Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation. Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals. Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny.

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References

    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. Science. 1988 Apr 8;240(4849):204-7 - PubMed
    1. Plant Mol Biol. 1994 Jan;24(2):317-25 - PubMed
    1. Plant Physiol. 1994 Jan;104(1):37-48 - PubMed
    1. Theor Appl Genet. 1991 Apr;81(4):437-44 - PubMed

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