Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Nov 14;39(5):925-38.
doi: 10.1016/j.immuni.2013.10.004. Epub 2013 Oct 31.

Origins and functional specialization of macrophages and of conventional and monocyte-derived dendritic cells in mouse skin

Samira Tamoutounour  1 , Martin Guilliams, Frédéric Montanana Sanchis, Hong Liu, Dorothea Terhorst, Camille Malosse, Emeline Pollet, Laurence Ardouin, Hervé Luche, Cindy Sanchez, Marc Dalod, Bernard Malissen, Sandrine Henri
Affiliations
Free article

Origins and functional specialization of macrophages and of conventional and monocyte-derived dendritic cells in mouse skin

Samira Tamoutounour et al. Immunity. .
Free article

Abstract

In the skin, the lack of markers permitting the unambiguous identification of macrophages and of conventional and monocyte-derived dendritic cells (DCs) complicates understanding of their contribution to skin integrity and to immune responses. By combining CD64 and CCR2 staining, we successfully identified each of these cell types and studied their origin, transcriptomic signatures, and migratory and T cell stimulatory properties. We also analyzed the impact of microbiota on their development and their contribution to skin inflammation during contact hypersensitivity. Dermal macrophages had a unique scavenging role and were unable to migrate and activate T cells. Conventional dermal DCs excelled both at migrating and activating T cells. In the steady-state dermis, monocyte-derived DCs are continuously generated by extravasated Ly-6C(hi) monocytes. Their T cell stimulatory capacity combined with their poor migratory ability made them particularly suited to activate skin-tropic T cells. Therefore, a high degree of functional specialization occurs among the mononuclear phagocytes of the skin.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

MeSH terms

Associated data