DNA determinants of rRNA synthesis in E. coli: growth rate dependent regulation, feedback inhibition, upstream activation, antitermination
- PMID: 2416474
- DOI: 10.1016/0092-8674(86)90498-8
DNA determinants of rRNA synthesis in E. coli: growth rate dependent regulation, feedback inhibition, upstream activation, antitermination
Abstract
We have examined the DNA regions required for rRNA synthesis in E. coli using promoter-lacZ and lambda PL-rrnB operon fusions. Sequences between -51 and -20 with respect to the P1 promoter transcription initiation site contain the critical information for growth rate dependent control. The region essential for growth rate regulation is the same as that necessary for feedback inhibition. A separate upstream region, between -51 and -88, increases rRNA transcription at least 15-fold and appears to have an abnormal conformation. The box A sequence downstream of promoter P2, but not DNA between P2 and box A, is required for efficient rRNA chain elongation. These results indicate that neither upstream activation nor antitermination determines growth rate dependence. Rather, growth rate regulation takes place at the target site for the negative feedback system, the P1 promoter itself. We propose that negative feedback regulation is responsible for the growth rate dependence of rRNA synthesis in E. coli.
Similar articles
-
Antitermination of E. coli rRNA transcription is caused by a control region segment containing lambda nut-like sequences.Cell. 1984 Oct;38(3):851-60. doi: 10.1016/0092-8674(84)90280-0. Cell. 1984. PMID: 6091902
-
Defective antitermination of rRNA transcription and derepression of rRNA and tRNA synthesis in the nusB5 mutant of Escherichia coli.Proc Natl Acad Sci U S A. 1985 Aug;82(16):5275-9. doi: 10.1073/pnas.82.16.5275. Proc Natl Acad Sci U S A. 1985. PMID: 3161080 Free PMC article.
-
Regulation of the Escherichia coli rrnB P2 promoter.J Bacteriol. 2003 Jan;185(1):28-34. doi: 10.1128/JB.185.1.28-34.2003. J Bacteriol. 2003. PMID: 12486037 Free PMC article.
-
Phage lambda and the regulation of transcription termination.Cell. 1988 Jan 15;52(1):5-6. doi: 10.1016/0092-8674(88)90523-5. Cell. 1988. PMID: 2449971 Review. No abstract available.
-
Antitermination mechanisms in rRNA operons of Escherichia coli.J Bacteriol. 1986 Oct;168(1):1-5. doi: 10.1128/jb.168.1.1-5.1986. J Bacteriol. 1986. PMID: 2428806 Free PMC article. Review. No abstract available.
Cited by
-
The tL structure within the leader region of Escherichia coli ribosomal RNA operons has post-transcriptional functions.Nucleic Acids Res. 1990 Jul 11;18(13):3893-901. doi: 10.1093/nar/18.13.3893. Nucleic Acids Res. 1990. PMID: 2197598 Free PMC article.
-
Deletions in the tL structure upstream to the rRNA genes in the E. coli rrnB operon cause transcription polarity.Nucleic Acids Res. 1987 Oct 26;15(20):8235-48. doi: 10.1093/nar/15.20.8235. Nucleic Acids Res. 1987. PMID: 3313279 Free PMC article.
-
Transcription of the tRNA-tufB operon of Escherichia coli: activation, termination and antitermination.Nucleic Acids Res. 1987 Nov 25;15(22):9515-30. doi: 10.1093/nar/15.22.9515. Nucleic Acids Res. 1987. PMID: 3317280 Free PMC article.
-
Bent DNA structures associated with several origins of replication are recognized by a unique enzyme from trypanosomatids.Nucleic Acids Res. 1988 Jul 25;16(14A):6477-92. doi: 10.1093/nar/16.14.6477. Nucleic Acids Res. 1988. PMID: 3399408 Free PMC article.
-
UP element-dependent transcription at the Escherichia coli rrnB P1 promoter: positional requirements and role of the RNA polymerase alpha subunit linker.Nucleic Acids Res. 2001 Oct 15;29(20):4166-78. doi: 10.1093/nar/29.20.4166. Nucleic Acids Res. 2001. PMID: 11600705 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
