doi: 10.1007/978-1-62703-670-2_9.
HIV-1 Rev function and RNA nuclear-cytoplasmic export
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- PMID: 24158817
- DOI: 10.1007/978-1-62703-670-2_9
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HIV-1 Rev function and RNA nuclear-cytoplasmic export
Methods Mol Biol.
2014.
Abstract
The requirement of HIV-1 and HTLV-1 to export incompletely spliced mRNAs has necessitated the evolution of Rev and Rex, respectively, to overcome host cellular mechanism that block nuclear-cytoplasmic export of incompletely processed mRNAs. Evaluating the function of these viral factors can be done at multiple levels: examining the functional consequence of Rev/Rex on viral gene expression, monitoring the movement of these proteins between the nucleus and cytoplasm or the subcellular distribution of the viral mRNAs. Here, I describe procedures to evaluate each of these aspects of Rev/Rex function.
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