Amino acid sequence of the alpha subunit of transducin deduced from the cDNA sequence
- PMID: 2409555
- PMCID: PMC390403
- DOI: 10.1073/pnas.82.13.4311
Amino acid sequence of the alpha subunit of transducin deduced from the cDNA sequence
Abstract
Transducin, a GTP-binding protein involved in phototransduction in the vertebrate retina, belongs to a family of homologous coupling proteins that also includes Gs and Gi, the regulatory proteins of adenylate cyclase. Here we report the cDNA sequence and deduced amino acid sequence of transducin's alpha subunit (T alpha). The cDNA was isolated, by screening with an antibody probe, from a bovine retinal cDNA library in the expression vector lambda gt11. The 2.2-kilobase cDNA insert hybridized to a single 2.6-kilobase poly(A)+ RNA species present in extracts of bovine retina but not of bovine heart, liver, or brain. The nucleotide sequence of the cDNA revealed an open reading frame long enough to encode the entire 39-kDa T alpha polypeptide. The polypeptide sequence deduced from the cDNA would be composed of 350 amino acids and have a molecular weight of 39,971. Portions of the sequence matched reported amino acid sequences of T alpha tryptic fragments, including sites specifically ADP-ribosylated by cholera and pertussis toxins. The predicted sequence also includes four segments, ranging from 11 to 19 residues in length, that exhibit significant homology to sequences of GTP-binding proteins, including the ras proteins of man and yeast and the elongation factors of ribosomal protein synthesis in bacteria, EF-G and EF-Tu. In combination with previous functional studies of tryptic fragments of T alpha, the deduced amino acid sequence makes it possible to predict which portions of the polypeptide interact with other molecules involved in retinal phototransduction.
Similar articles
-
Identification of the probable site of choleragen-catalyzed ADP-ribosylation in a Go alpha-like protein based on cDNA sequence.Proc Natl Acad Sci U S A. 1986 Aug;83(16):5813-6. doi: 10.1073/pnas.83.16.5813. Proc Natl Acad Sci U S A. 1986. PMID: 3090546 Free PMC article.
-
Primary structure of the beta-subunit of bovine transducin deduced from the cDNA sequence.FEBS Lett. 1985 Oct 28;191(2):235-40. doi: 10.1016/0014-5793(85)80015-6. FEBS Lett. 1985. PMID: 2414128
-
Primary structure of the alpha-subunit of bovine adenylate cyclase-inhibiting G-protein deduced from the cDNA sequence.FEBS Lett. 1986 Mar 3;197(1-2):305-10. doi: 10.1016/0014-5793(86)80347-7. FEBS Lett. 1986. PMID: 2419165
-
Sequence of the alpha subunit of photoreceptor G protein: homologies between transducin, ras, and elongation factors.Science. 1985 Apr 5;228(4695):96-9. doi: 10.1126/science.3856323. Science. 1985. PMID: 3856323
-
Structural and functional relationships of guanosine triphosphate binding proteins.Curr Top Cell Regul. 1988;29:129-216. doi: 10.1016/b978-0-12-152829-4.50006-9. Curr Top Cell Regul. 1988. PMID: 3135154 Review.
Cited by
-
Deduced primary structure of the alpha subunit of the GTP-binding stimulatory protein of adenylate cyclase.Proc Natl Acad Sci U S A. 1986 Mar;83(5):1251-5. doi: 10.1073/pnas.83.5.1251. Proc Natl Acad Sci U S A. 1986. PMID: 3081893 Free PMC article.
-
Domain mapping of the retinal cyclic GMP phosphodiesterase gamma-subunit. Function of the domains encoded by the three exons of the gamma-subunit gene.Biochem J. 1992 Feb 1;281 ( Pt 3)(Pt 3):637-43. doi: 10.1042/bj2810637. Biochem J. 1992. PMID: 1311170 Free PMC article.
-
Stimulation by nitric oxide of an NAD linkage to glyceraldehyde-3-phosphate dehydrogenase.Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6238-41. doi: 10.1073/pnas.90.13.6238. Proc Natl Acad Sci U S A. 1993. PMID: 8327504 Free PMC article.
-
A processed chicken pseudogene (CPS1) related to the ras oncogene superfamily.Nucleic Acids Res. 1986 Mar 11;14(5):2123-38. doi: 10.1093/nar/14.5.2123. Nucleic Acids Res. 1986. PMID: 3083400 Free PMC article.
-
Molecular cloning and sequence determination of cDNAs for alpha subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go from rat brain.Proc Natl Acad Sci U S A. 1986 Jun;83(11):3776-80. doi: 10.1073/pnas.83.11.3776. Proc Natl Acad Sci U S A. 1986. PMID: 3086867 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
