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. 2013 Nov:62:310-20.
doi: 10.1016/j.ijbiomac.2013.09.011. Epub 2013 Sep 20.

Chitosan-hyaluronic acid/nano silver composite sponges for drug resistant bacteria infected diabetic wounds

B S Anisha  1 , Raja Biswas, K P Chennazhi, R Jayakumar
Affiliations

Chitosan-hyaluronic acid/nano silver composite sponges for drug resistant bacteria infected diabetic wounds

B S Anisha et al. Int J Biol Macromol. 2013 Nov.

Abstract

The aim of this work was to develop an antimicrobial sponge composed of chitosan, hyaluronic acid (HA) and nano silver (nAg) as a wound dressing for diabetic foot ulcers (DFU) infected with drug resistant bacteria. nAg (5-20 nm) was prepared and characterized. The nanocomposite sponges were prepared by homogenous mixing of chitosan, HA and nAg followed by freeze drying to obtain a flexible and porous structure. The prepared sponges were characterized using SEM and FT-IR. The porosity, swelling, biodegradation and haemostatic potential of the sponges were also studied. Antibacterial activity of the prepared sponges was analysed using Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Klebsiella pneumonia. Chitosan-HA/nAg composite sponges showed potent antimicrobial property against the tested organisms. Sponges containing higher nAg (0.005%, 0.01% and 0.02%) concentrations showed antibacterial activity against MRSA. Cytotoxicity and cell attachment studies were done using human dermal fibroblast cells. The nanocomposite sponges showed a nAg concentration dependent toxicity towards fibroblast cells. Our results suggest that this nanocomposite sponges could be used as a potential material for wound dressing for DFU infected with antibiotic resistant bacteria if the optimal concentration of nAg exhibiting antibacterial action with least toxicity towards mammalian cells is identified.

Keywords: Chitosan–hyaluronic acid; Nano silver; Wound dressing-sponge-diabetic foot ulcers.

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