The interferon-inducible MxB protein inhibits HIV-1 infection
- PMID: 24055605
- DOI: 10.1016/j.chom.2013.08.015
The interferon-inducible MxB protein inhibits HIV-1 infection
Abstract
The interferon-inducible myxovirus resistance (Mx) proteins play important roles in combating a wide range of virus infections. MxA inhibits many RNA and DNA viruses, whereas the antiviral activity of MxB is less well established. We find that human MxB inhibits HIV-1 infection by reducing the level of integrated viral DNA. Passaging HIV-1 through MxB-expressing cells allowed the evolution of a mutant virus that escapes MxB restriction. HIV-1 escapes MxB restriction by mutating the alanine residue at position 88 in the viral capsid protein (CA), with a consequent loss of CA interaction with the host peptidylprolyl isomerase cyclophilin A (CypA), suggesting a role for CypA in MxB restriction. Consistent with this, MxB associates with CypA, and shRNA-mediated CypA depletion or cyclosporine A treatment resulted in the loss of MxB inhibition of HIV-1. Taken together, we conclude that human MxB protein inhibits HIV-1 DNA integration by a CypA-dependent mechanism.
Copyright © 2013 Elsevier Inc. All rights reserved.
Comment in
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Viral infection: interfering with HIV infection.Nat Rev Microbiol. 2013 Nov;11(11):742-3. doi: 10.1038/nrmicro3145. Epub 2013 Oct 8. Nat Rev Microbiol. 2013. PMID: 24100359 No abstract available.
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Dynamins are forever: MxB inhibits HIV-1.Cell Host Microbe. 2013 Oct 16;14(4):371-3. doi: 10.1016/j.chom.2013.10.002. Cell Host Microbe. 2013. PMID: 24139395
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