Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Mar:95 ( Pt 3):433-9.
doi: 10.1242/jcs.95.3.433.

Plasticity of the tubular lysosomal compartment in macrophages

P E Knapp  1 , J A Swanson
Affiliations

Plasticity of the tubular lysosomal compartment in macrophages

P E Knapp et al. J Cell Sci. 1990 Mar.

Abstract

Because bone marrow-derived macrophages differentiate in culture, their lysosomal compartment is largely devoid of the undigested particles that are common in macrophages removed from tissues. The morphology of this nearly vacant lysosomal compartment was observed, after labeling with fluorescent endocytic tracers such as Lucifer Yellow, to be an extensive, tubuloreticular network, which underwent extensive rearrangements in accommodating endocytic loads. It was converted to spherical organelles when the lysosomal compartment was loaded with osmotically active solutes such as sucrose or Acridine Orange. Enzymatic degradation of intravacuolar sucrose by pinocytosed invertase resulted in the shrinkage of vacuoles and the re-formation of the tubular network. After phagocytosis of opsonized erythrocytes or latex beads, tubular lysosomes wrapped around the phagosomes, then merged to form phagolysosomes. The disappearance of tubules was proportional to the total surface area of particles ingested. Degradation of the phagocytosed contents permitted shrinkage of the phagolysosome and concomitant re-formation of the tubuloreticular network. Nondegradable contents such as latex beads prevented re-formation of the tubular network. These rearrangements of the lysosomal compartment indicate that the organelle exhibits considerable plasticity and interconnectedness, and that maturation of lysosomes after endocytosis does not necessarily entail irreversible morphological changes.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

LinkOut - more resources