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. 1990 Jun;87(11):4033-7.
doi: 10.1073/pnas.87.11.4033.

Transferrin-polycation-mediated introduction of DNA into human leukemic cells: stimulation by agents that affect the survival of transfected DNA or modulate transferrin receptor levels

M Cotten  1 , F Längle-Rouault, H Kirlappos, E Wagner, K Mechtler, M Zenke, H Beug, M L Birnstiel
Affiliations

Transferrin-polycation-mediated introduction of DNA into human leukemic cells: stimulation by agents that affect the survival of transfected DNA or modulate transferrin receptor levels

M Cotten et al. Proc Natl Acad Sci U S A. 1990 Jun.

Abstract

We have subverted a receptor-mediated endocytosis event to transport genes into human leukemic cells. By coupling the natural iron-delivery protein transferrin to the DNA-binding polycations polylysine or protamine, we have created protein conjugates that bind nucleic acids and carry them into the cell during the normal transferrin cycle [Wagner, E., Zenke, M., Cotten, M., Beug, H. & Birnstiel, M. L. (1990) Proc. Natl. Acad. Sci. USA 87, 3410-3414]. We demonstrate here that this procedure is useful for a human leukemic cell line. We enhanced the rate of gene delivery by (i) increasing the transferrin receptor density through treatment of the cells with the cell-permeable iron chelator desferrioxamine, (ii) interfering with the synthesis of heme with succinyl acetone treatment, or (iii) stimulating the degradation of heme with cobalt chloride treatment. Consistent with gene delivery as an endocytosis event, we show that the subsequent expression in K-562 cells of a gene included in the transported DNA depends upon the cellular presence of the lysosomotropic agent chloroquine. By contrast, monensin blocks "transferrinfection," as does incubation of the cells at 18 degrees C.

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References

    1. Nucleic Acids Res. 1983 Mar 11;11(5):1295-308 - PubMed
    1. Int J Cancer. 1981 Mar 15;27(3):329-34 - PubMed
    1. J Cell Biol. 1983 Aug;97(2):329-39 - PubMed
    1. J Clin Pathol. 1984 Feb;37(2):131-5 - PubMed
    1. J Biol Chem. 1984 Mar 10;259(5):2689-92 - PubMed

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