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. 2011 Mar;23(1):43-8.
doi: 10.1007/s11670-011-0043-1.

Factors affecting mesenchymal stromal cells yield from bone marrow aspiration

Jing Li  1 , Wilfred Hing-Sang Wong, Shing Chan, James Chor-San Chim, Kenneth Man-Chee Cheung, Tsz-Leung Lee, Wing-Yan Au, Shau-Yin Ha, Albert Kwok-Wei Lie, Yu-Lung Lau, Raymond Hin-Suen Liang, Godfrey Chi-Fung Chan
Affiliations

Factors affecting mesenchymal stromal cells yield from bone marrow aspiration

Jing Li et al. Chin J Cancer Res. 2011 Mar.

Abstract

Objective: This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells (MSC).

Methods: WE DETERMINED THE YIELD OF MSC FROM BONE MARROW UNDER DIFFERENT CLINICAL CONDITIONS BY COMPARING THE MSC COLONY NUMBERS FROM: (1) donors of different ages; (2) healthy donors and patients with leukemia; (3) bone marrow aspirated at different time points during marrow harvesting; (4) bone marrow harvested by different needles.

Results: During the process of harvesting, the number of MSC significantly decreased with increase number of aspiration, from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated, and 50/ml after 200 ml bone marrow aspirated. The number of MSC retrieved from leukemia patients (99/ml bone marrow) was significantly lower than that of healthy donors (708/ml bone marrow). However, there was no significant difference in growth rate. There was no significant age-related difference of MSC yielded from donors <55 years. And there was no significant difference in MSC number between the samples from single end-holed needle and those from multiple-side-hole needle.

Conclusion: The optimal bone marrow samples for MSC collection should be obtained earlier in the process of harvesting procedure. Bone marrow from donors <55 years was equally good as MSC sources. The autologous MSC from leukemia patients can be utilized for in-vitro MSC expansion.

Keywords: Bone marrow aspiration; CFU-F; Leukemia; Mesenchymal stromal cell.

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Figures

Figure 1
Figure 1
Comparing conventional single end-holed needle (upper) and multiple side-holed DePuy Needle (lower).
Figure 2
Figure 2
Correlation of MSC colony with donors’ age and gender. MSC colony numbers of 29 donors were plotted against their age. No correlation between MSC colonies with age was noted.
Figure 3
Figure 3
MSC colony number from bone marrow of leukemia patients (n=21) and healthy donors (n=29). Results were represented as mean ±SEM of common logarithm transformation of MSC colony number. □ Represented common logarithm transformation of MSC colony per million MNC. ■ Represented common logarithm transformation of MSC colony per milliliter bone marrow. *P<0.05, **P<0.01.
Figure 4
Figure 4
Growth rate of MSC from leukemia patients (n=10) and healthy donors (n=7). Results were represented as mean ±SEM. There was no significant difference between the two groups.
Figure 5
Figure 5
MSC colony number from bone marrow samples aspirated by the multiple side-holed needle and the conventional end-holed needle (n=4). Results were represented as mean ±SEM. There was no significant difference between the two kinds of needles.
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References

    1. Fibbe WE, Noort WA. Mesenchymal stem cells and hematopoietic stem cell transplantation. Ann N Y Acad Sci 2003; 996:235-44 - PubMed
    1. Devine SM, Hoffman R. Role of mesenchymal stem cells in hematopoietic stem cell transplantation. Curr Opin Hematol 2000; 7: 358-63 - PubMed
    1. Aggarwal S, Pittenger MF. Human mesenchymal stem cells modulate allogeneic immune cell responses. Blood 2005; 105:1815-22 - PubMed
    1. Beyth S, Borovsky Z, Mevorach D, et al. Human mesenchymal stem cells alter antigen-presenting cell maturation and induce T-cell unresponsiveness. Blood 2005; 105:2214-9 - PubMed
    1. Chung NG, Jeong DC, Park SJ, et al. Cotransplantation of marrow stromal cells may prevent lethal graft-versus-host disease in major histocompatibility complex mismatched murine hematopoietic stem cell transplantation. Int J Hematol 2004; 80:370-6 - PubMed