Epidermal growth factor receptors increase during the differentiation of embryonal carcinoma cells
- PMID: 233127
- DOI: 10.1038/281309a0
Epidermal growth factor receptors increase during the differentiation of embryonal carcinoma cells
Abstract
Mouse teratocarcinoma stem cells (embryonal carcinoma, or EC cells) bind very small amounts of mouse epidermal growth factor (EGF) and the latter hormone seems to have no stimulatory effect on the growth of two cloned lines of EC cells. However, when EC cells are induced to differentiate into large flat endodern-like cells (END cells), EGF receptors increase in number reaching a plateau in 6 to 8 days. At 8 to 10 days after induction, END cells multiply very slowly, but when EGF is added (3 x 10(-10) M) to the medium, cell division is stimulated and a further change in morphology occurs. This letter describes the binding characteristics and numbers of the EGF receptors on EC and END cells and shows that exogenous retinoic acid increases the numbers of EGF receptors on END cells. We were unable to find endogenous competing factors produced by EC cells. Such factors could account for the lack of detectable binding of EGF on these cells. As EC cells differentiate to END cells, so the ability of the cells to form tumours is reduced. Since this change is accompanied by an increase in the number of EGF receptors there may be a relationship between these two events.
Similar articles
-
Epidermal growth factor receptors.Mol Cell Biochem. 1981 Feb 11;34(3):129-52. doi: 10.1007/BF02359619. Mol Cell Biochem. 1981. PMID: 6261114 Review.
-
Expression of EGF receptor and transferrin by F9 and PC13 teratocarcinoma cells.Differentiation. 1984;27(2):152-7. doi: 10.1111/j.1432-0436.1984.tb01421.x. Differentiation. 1984. PMID: 6090250
-
Acquisition of a limited lifespan by differentiating cells derived from PC13 embryonal carcinoma cells.J Cell Sci. 1984 Dec;72:227-40. doi: 10.1242/jcs.72.1.227. J Cell Sci. 1984. PMID: 6335886
-
Retinoids specifically enhance the number of epidermal growth factor receptors.Nature. 1980 Apr 17;284(5757):626-9. doi: 10.1038/284626a0. Nature. 1980. PMID: 6245371
-
Origin of mouse embryonal carcinoma cells and the possibility of their direct isolation into tissue culture.J Reprod Fertil. 1981 Jul;62(2):625-31. doi: 10.1530/jrf.0.0620625. J Reprod Fertil. 1981. PMID: 7019433 Review. No abstract available.
Cited by
-
Expression of fibroblast growth factor by F9 teratocarcinoma cells as a function of differentiation.J Cell Biol. 1989 Jun;108(6):2467-76. doi: 10.1083/jcb.108.6.2467. J Cell Biol. 1989. PMID: 2544608 Free PMC article.
-
Epidermal growth factor receptors.Mol Cell Biochem. 1981 Feb 11;34(3):129-52. doi: 10.1007/BF02359619. Mol Cell Biochem. 1981. PMID: 6261114 Review.
-
Fibrinogen mitogenic effect on hemopoietic cell lines: control via receptor modulation.Proc Natl Acad Sci U S A. 1986 Sep;83(17):6494-8. doi: 10.1073/pnas.83.17.6494. Proc Natl Acad Sci U S A. 1986. PMID: 3018735 Free PMC article.
-
Isolation of cDNA clones for genes exhibiting reduced expression after differentiation of murine teratocarcinoma stem cells.Mol Cell Biol. 1984 Oct;4(10):2142-50. doi: 10.1128/mcb.4.10.2142-2150.1984. Mol Cell Biol. 1984. PMID: 6095043 Free PMC article.
-
A rapid decrease in epidermal growth factor-binding capacity accompanies the terminal differentiation of mouse myoblasts in vitro.J Cell Biol. 1984 Feb;98(2):739-47. doi: 10.1083/jcb.98.2.739. J Cell Biol. 1984. PMID: 6319433 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
