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Comparative Study
. 2013 Apr;34(2):983-6.
doi: 10.1007/s13277-012-0634-6. Epub 2012 Dec 27.

Characterization of cell-free circulating DNA in plasma in patients with prostate cancer

Pâmela Oliveira Delgado  1 , Beatriz Costa A Alves, Flávia de Sousa Gehrke, Renata Kelly Kuniyoshi, Marcelo Langer Wroclavski, Auro Del Giglio, Fernando Luiz Affonso Fonseca
Affiliations
Comparative Study

Characterization of cell-free circulating DNA in plasma in patients with prostate cancer

Pâmela Oliveira Delgado et al. Tumour Biol. 2013 Apr.

Abstract

Cell-free circulating DNA in plasma and serum may serve as a biomarker for malignant tumor detection and follow up in patients with a variety of solid tumors including prostate cancer. In healthy patients, DNA is normally released from an apoptotic source which generates small fragments of cell-free DNA, whereas cancer patients have cell-free circulating DNA that originated from necrosis, autophagy, or mitotic catastrophe. Cell-free circulating DNA levels were measured by a quantitative real-time PCR method with a set of primers targeted to amplify the consensus ALU apoptotic versus necrotic origin. Prostate cancer patients before and 3 months after diagnosis showed cell-free circulating DNA released at apoptotic and non-apoptotic cell death. Interestingly, all patients after 6 months demonstrated DNA released at non-apoptotic cell. The principal source of cell-free circulating DNA is of apoptotic and non-apoptotic cell death. However, during treatment, this feature could change. Therefore, the study of cell-free circulating DNA would be important to follow the evolution of the disease during the treatment.

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