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. 1990 Apr;87(8):3170-3.
doi: 10.1073/pnas.87.8.3170.

Constructing deletions with defined endpoints in Drosophila

L Cooley  1 , D Thompson, A C Spradling
Affiliations

Constructing deletions with defined endpoints in Drosophila

L Cooley et al. Proc Natl Acad Sci U S A. 1990 Apr.

Abstract

Chromosomes bearing small deletions are valuable tools in Drosophila genetics. We have investigated a method for efficiently constructing precise chromosomal deficiencies. Two P transposable elements were positioned within a progenitor strain at the sites of the desired deletion endpoints. Deletions spanning the two transposons were recovered at high frequency when P element transposase was expressed in these flies, but only if the flanking P elements were in a cis rather than a trans configuration. Appropriate progenitor strains can now be constructed to delete virtually any chromosomal region by utilizing an extensive collection of lines containing single P element insertions throughout the Drosophila genome.

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