Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Feb 5;85(3):1501-8.
doi: 10.1021/ac302629u. Epub 2013 Jan 9.

Cu(II) affinity for the Alzheimer's peptide: tyrosine fluorescence studies revisited

Bruno Alies  1 , Emelyne Renaglia, Malgorzata Rózga, Wojciech Bal, Peter Faller, Christelle Hureau
Affiliations

Cu(II) affinity for the Alzheimer's peptide: tyrosine fluorescence studies revisited

Bruno Alies et al. Anal Chem. .

Abstract

Copper(II) binding to the amyloid-β peptide has been proposed to be a key event in the cascade leading to Alzheimer's disease. As a direct consequence, the strength of the Cu(II) to Aβ interaction, that is, the Cu(II) affinity of Aβ, is a very important parameter to determine. Because Aβ peptide contain one Tyr fluorophore in its sequence and because Cu(II) does quench Tyr fluorescence, fluorescence measurements appear to be a straightforward way to obtain this parameter. However, this proved to be wrong, mainly because of data misinterpretation in some previous studies that leads to a conflicting situation. In the present paper, we have investigated in details a large set of fluorescence data that were analyzed with a new method taking into account the presence of two Cu(II) sites and the inner-filter effect. This leads to reinterpretation of the published data and to the determination of a unified affinity value in the 10(10) M(-1) range.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

LinkOut - more resources