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. 2012 Oct;50(5):837-41.
doi: 10.1007/s12275-012-2468-8. Epub 2012 Nov 4.

Intestinal intraepithelial TCRγδ⁺ T cells are activated by normal commensal bacteria

Sang Phil Jeong  1 , Jung-Ah Kang, Sung-Gyoo Park
Affiliations

Intestinal intraepithelial TCRγδ⁺ T cells are activated by normal commensal bacteria

Sang Phil Jeong et al. J Microbiol. 2012 Oct.

Abstract

TCRγδ(+) T cells play a critical role in protecting the intestinal mucosa against pathogenic infection. In the absence of infection, TCRγδ(+) T cell activation must be continuously regulated by T regulatory cells (Treg) to prevent the development of colitis. However, the activation of intestinal TCRγδ(+) T cells under normal conditions has not been clearly resolved. In order to determine TCRγδ(+) T cell activation in vivo, we designed an NF-κB based reporter system. Using the recombinant lentiviral method, we delivered the NF-κB reporter to isolated TCRγδ(+) T cells, which were then adoptively transferred into normal mice. Our data indicate that the NF-κB activation level in TCRγδ(+) T cells is higher in the intestinal intraepithelial layer than in the lamina propria region. In addition, the surface expression level of lymphocyte activation marker CD69 in TCRγδ(+) T cells is also higher in the intestinal intraepithelial layer and this activation was reduced by Sulfatrim treatment which removes of commensal bacteria. Collectively, our data indicate that the TCRγδ(+) T cell population attached to the intestinal lumen is constitutively activated even by normal commensal bacteria.

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