The size of chromatin loops in HeLa cells
- PMID: 2303042
- PMCID: PMC551702
- DOI: 10.1002/j.1460-2075.1990.tb08144.x
The size of chromatin loops in HeLa cells
Abstract
It is widely believed that the chromatin fibre is organized into loops during interphase, with the loop being implicated as an important unit of nuclear function. However, there remains little direct evidence for looping, with estimates of loop size varying widely. This has led to the suggestion that some loops, or even all of them, arise artefactually during isolation as chromatin aggregates so easily. We have now investigated the effect of isolation procedure on loop size using HeLa cells encapsulated in agarose to allow easy manipulation. Loop size in various derivatives (i.e. nuclei, nucleoids, matrices and scaffolds) critically depended on procedure; some (or all) of their loops are artefacts. The loop size in derivatives isolated using the most 'physiological' conditions was 86 kb; this remained unchanged throughout the cell cycle. This loop size is probably an average of a range of loops of between 5 and 200 kb.
Similar articles
-
Isolation and partial characterization of the nuclear shell of HeLa cells.Exp Cell Res. 1981 Feb;131(2):446-52. doi: 10.1016/0014-4827(81)90254-8. Exp Cell Res. 1981. PMID: 7202548 No abstract available.
-
Replication occurs at a nucleoskeleton.EMBO J. 1986 Jun;5(6):1403-10. doi: 10.1002/j.1460-2075.1986.tb04374.x. EMBO J. 1986. PMID: 3732249 Free PMC article.
-
The nuclear shell in HeLa cell nuclei: whole-mount electron microscopy of the dissociated and isolated nuclear periphery.J Ultrastruct Res. 1980 Dec;73(3):288-98. doi: 10.1016/s0022-5320(80)90088-x. J Ultrastruct Res. 1980. PMID: 7230321 No abstract available.
-
Simulation of different three-dimensional polymer models of interphase chromosomes compared to experiments-an evaluation and review framework of the 3D genome organization.Semin Cell Dev Biol. 2019 Jun;90:19-42. doi: 10.1016/j.semcdb.2018.07.012. Epub 2018 Aug 24. Semin Cell Dev Biol. 2019. PMID: 30125668 Review.
-
Heterogeneity and territorial organization of the nuclear matrix and related structures.Int Rev Cytol. 1983;83:135-82. doi: 10.1016/s0074-7696(08)61687-3. Int Rev Cytol. 1983. PMID: 6358101 Review. No abstract available.
Cited by
-
Attachment of DNA to the nucleoskeleton of HeLa cells examined using physiological conditions.Nucleic Acids Res. 1990 Aug 11;18(15):4385-93. doi: 10.1093/nar/18.15.4385. Nucleic Acids Res. 1990. PMID: 2167466 Free PMC article.
-
Use of matrix attachment regions (MARs) to minimize transgene silencing.Plant Mol Biol. 2000 Jun;43(2-3):361-76. doi: 10.1023/a:1006424621037. Plant Mol Biol. 2000. PMID: 10999416 Review.
-
Micromechanical studies of mitotic chromosomes.J Muscle Res Cell Motil. 2002;23(5-6):409-31. J Muscle Res Cell Motil. 2002. PMID: 12785094 Review.
-
Transcriptome profiling of human hepatocytes treated with Aroclor 1254 reveals transcription factor regulatory networks and clusters of regulated genes.BMC Genomics. 2006 Aug 26;7:217. doi: 10.1186/1471-2164-7-217. BMC Genomics. 2006. PMID: 16934159 Free PMC article.
-
Dynamics of nuclear matrix attachment regions during 5th instar posterior silk gland development in Bombyx mori.BMC Genomics. 2022 Mar 31;23(1):247. doi: 10.1186/s12864-022-08446-3. BMC Genomics. 2022. PMID: 35361117 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources