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. 2012 Oct 18;490(7420):367-72.
doi: 10.1038/nature11442. Epub 2012 Sep 23.

Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F

Vladimir M Korkhov  1 , Samantha A Mireku, Kaspar P Locher
Affiliations

Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F

Vladimir M Korkhov et al. Nature. .

Abstract

The ATP-binding cassette (ABC) transporter BtuCD mediates the uptake of vitamin B(12) across the inner membrane of Escherichia coli. Previous structures have shown the conformations of apo states, but the transport mechanism has remained unclear. Here we report the 3.5 Å crystal structure of the transporter-binding protein complex BtuCD-BtuF (BtuCD-F) trapped in an β-γ-imidoadenosine 5'-phosphate (AMP-PNP)-bound intermediate state. Although the ABC domains (BtuD subunits) form the expected closed sandwich dimer, the membrane-spanning BtuC subunits adopt a new conformation, with the central translocation pathway sealed by a previously unrecognized cytoplasmic gate. A fully enclosed cavity is thus formed approximately halfway across the membrane. It is large enough to accommodate a vitamin B(12) molecule, and radioligand trapping showed that liposome-reconstituted BtuCD-F indeed contains bound B(12) in the presence of AMP-PNP. In combination with engineered disulphide crosslinking and functional assays, our data suggest an unexpected peristaltic transport mechanism that is distinct from those observed in other ABC transporters.

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References

    1. Science. 2007 Jan 19;315(5810):373-7 - PubMed
    1. Mol Cell. 2006 Oct 6;24(1):51-62 - PubMed
    1. Mol Cell. 2002 Jul;10(1):139-49 - PubMed
    1. J Mol Biol. 2007 Oct 5;372(5):1179-88 - PubMed
    1. Nat Rev Mol Cell Biol. 2009 Mar;10(3):218-27 - PubMed

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