Restriction endonuclease mapping of unintegrated proviral DNA of Snyder-Theilen feline sarcoma virus: localization of sarcoma-specific sequences
- PMID: 229270
- PMCID: PMC525935
- DOI: 10.1128/JVI.32.3.860-875.1979
Restriction endonuclease mapping of unintegrated proviral DNA of Snyder-Theilen feline sarcoma virus: localization of sarcoma-specific sequences
Abstract
Extrachromosomal DNA purified from mink cells acutely infected with the Snyder-Theilen strain of feline sarcoma virus (FeSV) was digested with restriction endonucleases, and the DNA fragments were electrophoretically separated, transferred to a solid substrate, and hybridized with radiolabeled DNA transcripts complementary to different portions of the FeSV RNA genome. Major DNA species 8.4 and 5.0 kilobase pairs (kbp) long represent the linear, unintegrated proviruses of Snyder-Theilen feline leukemia virus and FeSV, respectively. Transfection experiments performed with electroeluted DNAs showed that the 8.4-kbp form led to the production of replicating nontransforming virus in mink and cat cells; in contrast, the 5.0-kbp DNA produced helper virus-independent foci of transformation in mouse NIH/3T3 cells and helper virus-dependent foci in mink cells at an efficiency comparable to that obtained with unfractionated extrachromosomal DNA. Sites of restriction endonuclease cleavage for six enzymes were oriented with respect to one another within the FeSV provirus. EcoRI recognized cleavage sites at 0.3 to 0.4 kbp from each terminus of FeSV DNA, reducing the 5.0-kbp DNA to molecules 4.3 kbp long; this enzyme excised a large internal proviral DNA fragment of corresponding size from the DNA of FeSV-transformed mink nonproducer cells. By using DNA transcripts complementary to different portions of the FeSV genome, sarcoma-specific sequences (the FeSV src gene) were positioned within 2.1 and 3.4 kbp from the 5' end of the proviral DNA with respect to the viral RNA genome. The src gene is flanked at both ends by sequences shared in common with feline leukemia virus. The localization of src sequences to this region suggests that a portion of an FeSV polyprotein which contains feline oncornavirus-associated cell membrane antigen (FOCMA-S) is the major product of this gene.
Similar articles
-
McDonough feline sarcoma virus: characterization of the molecularly cloned provirus and its feline oncogene (v-fms).J Virol. 1982 Feb;41(2):489-500. doi: 10.1128/JVI.41.2.489-500.1982. J Virol. 1982. PMID: 6281462 Free PMC article.
-
Molecular cloning of Snyder-Theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus.J Virol. 1980 Apr;34(1):200-12. doi: 10.1128/JVI.34.1.200-212.1980. J Virol. 1980. PMID: 6246254 Free PMC article.
-
Cellular transformation by subgenomic feline sarcoma virus DNA.J Virol. 1981 Jan;37(1):518-23. doi: 10.1128/JVI.37.1.518-523.1981. J Virol. 1981. PMID: 6261000 Free PMC article.
-
Silencing of Unintegrated Retroviral DNAs.Viruses. 2021 Nov 9;13(11):2248. doi: 10.3390/v13112248. Viruses. 2021. PMID: 34835055 Free PMC article. Review.
-
Overlooked: Extrachromosomal DNA and Their Possible Impact on Whole Genome Sequencing.Malays J Med Sci. 2018 Mar;25(2):20-26. doi: 10.21315/mjms2018.25.2.3. Epub 2018 Apr 27. Malays J Med Sci. 2018. PMID: 30918452 Free PMC article. Review.
Cited by
-
Transfer and expression of the gene encoding a human myeloid membrane antigen (gp150).J Clin Invest. 1985 Feb;75(2):569-79. doi: 10.1172/JCI111733. J Clin Invest. 1985. PMID: 3973018 Free PMC article.
-
Transformation-defective mutants of feline sarcoma virus which express a product of the viral src gene.J Virol. 1980 Jul;35(1):129-40. doi: 10.1128/JVI.35.1.129-140.1980. J Virol. 1980. PMID: 6251261 Free PMC article.
-
Fujinami sarcoma virus: an avian RNA tumor virus with a unique transforming gene.Proc Natl Acad Sci U S A. 1980 Apr;77(4):2018-22. doi: 10.1073/pnas.77.4.2018. Proc Natl Acad Sci U S A. 1980. PMID: 6246518 Free PMC article.
-
Clonal analysis of early and late stages of erythroleukemia induced by molecular clones of integrated spleen focus-forming virus.Proc Natl Acad Sci U S A. 1981 Nov;78(11):6893-7. doi: 10.1073/pnas.78.11.6893. Proc Natl Acad Sci U S A. 1981. PMID: 6273894 Free PMC article.
-
Comparative restriction endonuclease maps of proviral DNA of the primate type C simian sarcoma-associated virus and gibbon ape leukemia virus group.J Virol. 1982 Jan;41(1):298-308. doi: 10.1128/JVI.41.1.298-308.1982. J Virol. 1982. PMID: 6283121 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous