Restriction endonuclease mapping of unintegrated proviral DNA of Snyder-Theilen feline sarcoma virus: localization of sarcoma-specific sequences
- PMID: 229270
- PMCID: PMC525935
- DOI: 10.1128/JVI.32.3.860-875.1979
Restriction endonuclease mapping of unintegrated proviral DNA of Snyder-Theilen feline sarcoma virus: localization of sarcoma-specific sequences
Abstract
Extrachromosomal DNA purified from mink cells acutely infected with the Snyder-Theilen strain of feline sarcoma virus (FeSV) was digested with restriction endonucleases, and the DNA fragments were electrophoretically separated, transferred to a solid substrate, and hybridized with radiolabeled DNA transcripts complementary to different portions of the FeSV RNA genome. Major DNA species 8.4 and 5.0 kilobase pairs (kbp) long represent the linear, unintegrated proviruses of Snyder-Theilen feline leukemia virus and FeSV, respectively. Transfection experiments performed with electroeluted DNAs showed that the 8.4-kbp form led to the production of replicating nontransforming virus in mink and cat cells; in contrast, the 5.0-kbp DNA produced helper virus-independent foci of transformation in mouse NIH/3T3 cells and helper virus-dependent foci in mink cells at an efficiency comparable to that obtained with unfractionated extrachromosomal DNA. Sites of restriction endonuclease cleavage for six enzymes were oriented with respect to one another within the FeSV provirus. EcoRI recognized cleavage sites at 0.3 to 0.4 kbp from each terminus of FeSV DNA, reducing the 5.0-kbp DNA to molecules 4.3 kbp long; this enzyme excised a large internal proviral DNA fragment of corresponding size from the DNA of FeSV-transformed mink nonproducer cells. By using DNA transcripts complementary to different portions of the FeSV genome, sarcoma-specific sequences (the FeSV src gene) were positioned within 2.1 and 3.4 kbp from the 5' end of the proviral DNA with respect to the viral RNA genome. The src gene is flanked at both ends by sequences shared in common with feline leukemia virus. The localization of src sequences to this region suggests that a portion of an FeSV polyprotein which contains feline oncornavirus-associated cell membrane antigen (FOCMA-S) is the major product of this gene.
Similar articles
-
Molecular cloning of Snyder-Theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus.J Virol. 1980 Apr;34(1):200-12. doi: 10.1128/JVI.34.1.200-212.1980. J Virol. 1980. PMID: 6246254 Free PMC article.
-
Cellular transformation by subgenomic feline sarcoma virus DNA.J Virol. 1981 Jan;37(1):518-23. doi: 10.1128/JVI.37.1.518-523.1981. J Virol. 1981. PMID: 6261000 Free PMC article.
-
McDonough feline sarcoma virus: characterization of the molecularly cloned provirus and its feline oncogene (v-fms).J Virol. 1982 Feb;41(2):489-500. doi: 10.1128/JVI.41.2.489-500.1982. J Virol. 1982. PMID: 6281462 Free PMC article.
-
Silencing of Unintegrated Retroviral DNAs.Viruses. 2021 Nov 9;13(11):2248. doi: 10.3390/v13112248. Viruses. 2021. PMID: 34835055 Free PMC article. Review.
-
Overlooked: Extrachromosomal DNA and Their Possible Impact on Whole Genome Sequencing.Malays J Med Sci. 2018 Mar;25(2):20-26. doi: 10.21315/mjms2018.25.2.3. Epub 2018 Apr 27. Malays J Med Sci. 2018. PMID: 30918452 Free PMC article. Review.
Cited by
-
Molecular cloning of Snyder-Theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus.J Virol. 1980 Apr;34(1):200-12. doi: 10.1128/JVI.34.1.200-212.1980. J Virol. 1980. PMID: 6246254 Free PMC article.
-
Cellular transformation by subgenomic feline sarcoma virus DNA.J Virol. 1981 Jan;37(1):518-23. doi: 10.1128/JVI.37.1.518-523.1981. J Virol. 1981. PMID: 6261000 Free PMC article.
-
A recessive cellular mutation in v-fes-transformed mink cells restores contact inhibition and anchorage-dependent growth.Mol Cell Biol. 1988 Jun;8(6):2419-27. doi: 10.1128/mcb.8.6.2419-2427.1988. Mol Cell Biol. 1988. PMID: 3261387 Free PMC article.
-
Molecular cloning, expression, and chromosomal localization of the gene encoding a human myeloid membrane antigen (gp150).J Clin Invest. 1986 Oct;78(4):914-21. doi: 10.1172/JCI112680. J Clin Invest. 1986. PMID: 2428842 Free PMC article.
-
Immunopathology induced by the feline leukemia virus.Springer Semin Immunopathol. 1982;5(1):75-106. doi: 10.1007/BF00201958. Springer Semin Immunopathol. 1982. PMID: 6195744 Review. No abstract available.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous