Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1990;8(3):237-50.
doi: 10.1002/prot.340080306.

Generation of potential structures for the G-domain of chloroplast EF-Tu using comparative molecular modeling

M A Lapadat  1 , D W Deerfield 2nd, L G Pedersen, L L Spremulli
Affiliations
Comparative Study

Generation of potential structures for the G-domain of chloroplast EF-Tu using comparative molecular modeling

M A Lapadat et al. Proteins. 1990.

Abstract

Comparative molecular modeling has been used to generate several possible structures for the G-domain of chloroplast elongation factor Tu (EF-Tu(chl)) based on the crystallographic data of the homologous E. coli protein. EF-Tu(chl) contains a 10 amino acid insertion not present in the E. coli protein and this region has been modeled based on its predicted secondary structure. The insertion appears to lie on the surface of the protein. Its orientation could not be determined unequivocally but several likely structures for the nucleotide binding domain of EF-Tu(chl) have been developed. The effects of the presence of water in the Mg2+ coordination sphere and of the protonation state of the GDP ligand on the conformation of the guanine nucleotide binding site have been examined. Relative binding constants of several guanine nucleotide analogs for EF-Tu(chl) have been obtained. The interactions between EF-Tu(chl) and GDP predicted to be important by the models that have been developed are discussed in relation to the nucleotide binding properties of this factor and to the interactions proposed to be important in the binding of guanine nucleotides to related proteins.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

  • Extrachromosomal DNA in the Apicomplexa.
    Wilson RJ, Williamson DH. Wilson RJ, et al. Microbiol Mol Biol Rev. 1997 Mar;61(1):1-16. doi: 10.1128/mmbr.61.1.1-16.1997. Microbiol Mol Biol Rev. 1997. PMID: 9106361 Free PMC article. Review.
  • Chloroplast ribosomes and protein synthesis.
    Harris EH, Boynton JE, Gillham NW. Harris EH, et al. Microbiol Rev. 1994 Dec;58(4):700-54. doi: 10.1128/mr.58.4.700-754.1994. Microbiol Rev. 1994. PMID: 7854253 Free PMC article. Review.

Publication types

LinkOut - more resources