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. 2012 Aug 13;13(8):2219-24.
doi: 10.1021/bm300646q. Epub 2012 Jul 10.

Photocontrolled nanoparticles for on-demand release of proteins

Malar A Azagarsamy  1 , Daniel L Alge, Srinidhi J Radhakrishnan, Mark W Tibbitt, Kristi S Anseth
Affiliations
Free PMC article

Photocontrolled nanoparticles for on-demand release of proteins

Malar A Azagarsamy et al. Biomacromolecules. .
Free PMC article

Abstract

We describe here light-regulated swelling and degradation features of polymeric nanoparticles that are produced using an inverse microemulsion polymerization method. We demonstrate the phototriggered release characteristics of the nanoparticles by sequestering protein molecules and releasing them using light as a trigger. Furthermore, the intracellular translocation of the nanoparticles, along with its fluorescent protein payload, was achieved using a cell-penetrating peptide-based surface modification. We expect that the noncovalent encapsulation of proteins using nanoparticles and their photo triggered release using an external light would provide opportunities for achieving intracellular release of molecular therapeutics for on-demand requirements.

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Figures

Figure 1
Figure 1
(a) Schematic illustration of protein encapsulation and its light-induced release from cross-linked polymeric nanogels. (b) Chemical structures of monomers used in the synthesis of photocontrolled nanogels. (c) TEM image of nanogels. (d) Hydrodynamic size of nanogel (0.2 mg/mL).
Scheme 1
Scheme 1. (a) Synthesis of Key Photocleavable Crosslinker A. (b) Light Induced De-cross-linking of Photodegradable Nanogel
Figure 2
Figure 2
Studies of photoresponsive properties and protein release: (a) Change in hydrodynamic size of nanogel (0.2 mg/mL) at different time of light exposures (2.5, 5 and 10 min) using DLS; (b) GPC traces outlining light induced degradation of nanogel (1.0 mg/mL); (c) Control over protein activity: Evolution of p-NP (λmax: 400 nm) upon exposing p-NPP substrate (0.2 mM) to ALP loaded nanogel (0.2 mg/mL) and its light exposed (2 min of exposure) counterpart in UV–vis; (d) SDS-PAGE electrophoresis depicting protein release: L - ladder; 1- native ALP; 2- ALP loaded nanogel; 3- ALP loaded nanogel after exposure to light (2 min); 4 - blank nanogel (not loaded with ALP).
Figure 3
Figure 3
Confocal images of 3T3 cells after being exposed to flourescein-BSA loaded nanogel: (a) without TAT peptide; (b) with TAT peptide.
See this image and copyright information in PMC

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