Extrapulmonary locations of mycobacterium tuberculosis DNA during latent infection
- PMID: 22732919
- DOI: 10.1093/infdis/jis381
Extrapulmonary locations of mycobacterium tuberculosis DNA during latent infection
Abstract
Background: One-third of the world's population has latent infection with Mycobacterium tuberculosis, and 10%-15% of cases of reactivation occur at extrapulmonary sites without active pulmonary tuberculosis.
Methods: To establish the frequency and location of mycobacterial DNA, organ specimens from 49 individuals who died from causes other than tuberculosis were studied by means of polymerase chain reaction (PCR), PCR plus DNA hybridization, in situ PCR, real-time PCR, and spoligotyping.
Results: Lung specimens from most subjects (36) were positive for M. tuberculosis, as were specimens from the spleen (from 35 subjects), kidney (from 34), and liver (from 33). By in situ PCR, mycobacterial DNA was found in endothelium, pneumocytes, and macrophages from the lung and in Bowman's parietal cells and convoluted proximal tubules from the kidney. In spleen, macrophages and sinusoidal endothelial cells were positive, whereas in liver, Kupffer cells and sinusoidal endothelium were commonly positive. Spoligotyping of 54 pulmonary and extrapulmonary positive tissues from 30 subjects showed 43 different genotypes, including 36 orphan types. To confirm the viability of mycobacteria, 10 positive tissue samples were selected for isolation of mycobacterial RNA. All samples showed 16S ribosomal RNA expression, while 8 and 4 samples showed expression of the latent infection genes encoding isocitrate lyase and α-crystallin, respectively.
Conclusions: M. tuberculosis persists in several sites and cell types that might constitute reservoirs that can reactivate infection, producing extrapulmonary tuberculosis without lung involvement.
Comment in
-
How does Mycobacterium tuberculosis establish infection?J Infect Dis. 2012 Oct;206(8):1157-9. doi: 10.1093/infdis/jis382. Epub 2012 Jun 25. J Infect Dis. 2012. PMID: 22732920 No abstract available.
Similar articles
-
Multiplex PCR assay for simultaneous detection and differentiation of Mycobacterium tuberculosis, Mycobacterium avium complexes and other Mycobacterial species directly from clinical specimens.J Appl Microbiol. 2009 Aug;107(2):425-35. doi: 10.1111/j.1365-2672.2009.04218.x. Epub 2009 Mar 16. J Appl Microbiol. 2009. PMID: 19302308
-
Persistence of DNA from Mycobacterium tuberculosis in superficially normal lung tissue during latent infection.Lancet. 2000 Dec 23-30;356(9248):2133-8. doi: 10.1016/s0140-6736(00)03493-0. Lancet. 2000. PMID: 11191539
-
Is adipose tissue a place for Mycobacterium tuberculosis persistence?PLoS One. 2006 Dec 20;1(1):e43. doi: 10.1371/journal.pone.0000043. PLoS One. 2006. PMID: 17183672 Free PMC article.
-
A review of murine models of latent tuberculosis infection.Scand J Infect Dis. 2011 Dec;43(11-12):848-56. doi: 10.3109/00365548.2011.603745. Epub 2011 Sep 6. Scand J Infect Dis. 2011. PMID: 21892898 Review.
-
Genomic plasticity between human and mycobacterial DNA: A review.Tuberculosis (Edinb). 2017 Dec;107:38-47. doi: 10.1016/j.tube.2017.03.006. Epub 2017 Aug 9. Tuberculosis (Edinb). 2017. PMID: 29050770 Review.
Cited by
-
Next-Gen Dual Transcriptomics for Adult Extrapulmonary Tuberculosis Biomarkers and Host-Pathogen Interplay in Human Cells: A Strategic Review.Indian J Microbiol. 2024 Mar;64(1):36-47. doi: 10.1007/s12088-023-01143-z. Epub 2023 Dec 14. Indian J Microbiol. 2024. PMID: 38468742 Free PMC article. Review.
-
High Genotypic Discordance of Concurrent Mycobacterium tuberculosis Isolates from Sputum and Blood of HIV-Infected Individuals.PLoS One. 2015 Jul 15;10(7):e0132581. doi: 10.1371/journal.pone.0132581. eCollection 2015. PLoS One. 2015. PMID: 26176604 Free PMC article.
-
Diagnosis of human immunodeficiency virus associated disseminated intravascular coagulation.PLoS One. 2022 Jan 21;17(1):e0262306. doi: 10.1371/journal.pone.0262306. eCollection 2022. PLoS One. 2022. PMID: 35061794 Free PMC article.
-
The Mtb-HIV syndemic interaction: why treating M. tuberculosis infection may be crucial for HIV-1 eradication.Future Virol. 2020 Feb;15(2):101-125. doi: 10.2217/fvl-2019-0069. Future Virol. 2020. PMID: 32273900 Free PMC article. Review.
-
The in vivo environment accelerates generation of resuscitation-promoting factor-dependent mycobacteria.Am J Respir Crit Care Med. 2014 Dec 15;190(12):1455-7. doi: 10.1164/rccm.201407-1289LE. Am J Respir Crit Care Med. 2014. PMID: 25496107 Free PMC article. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources