Rab11 regulates exocytosis of recycling vesicles at the plasma membrane
- PMID: 22685325
- DOI: 10.1242/jcs.102913
Rab11 regulates exocytosis of recycling vesicles at the plasma membrane
Abstract
Rab11 is known to associate primarily with perinuclear recycling endosomes and regulate recycling of endocytosed proteins. However, the recycling step in which Rab11 participates remains unknown. We show here that, in addition to causing tubulation of recycling endosomes, Rab11 depletion gives rise to accumulation of recycling carriers containing endocytosed transferrin and transferrin receptor beneath the plasma membrane. We also show that the carriers are transported from perinuclear recycling endosomes to the cell periphery along microtubules. Total internal reflection fluorescence microscopy of cells expressing EGFP-tagged transferrin receptor revealed that Rab11 depletion inhibits tethering and fusion of recycling carriers to the plasma membrane. Depletion of Sec15, which interacts with Rab11, or Exo70, both components of the exocyst tethering complex, leads to essentially the same phenotypes as those of Rab11 depletion. Thus, in addition to its role in recycling processes at perinuclear recycling endosomes, Rab11 is transported along microtubules to the cell periphery through association with recycling carriers, and directly regulates vesicle exocytosis at the plasma membrane in concert with the exocyst.
Similar articles
-
ARF1 and ARF3 are required for the integrity of recycling endosomes and the recycling pathway.Cell Struct Funct. 2012;37(2):141-54. doi: 10.1247/csf.12015. Epub 2012 Sep 5. Cell Struct Funct. 2012. PMID: 22971977
-
Reconstitution of vesicular transport to Rab11-positive recycling endosomes in vitro.Biochem Biophys Res Commun. 2003 Dec 19;312(3):663-9. doi: 10.1016/j.bbrc.2003.10.172. Biochem Biophys Res Commun. 2003. PMID: 14680816
-
GTP hydrolysis of TC10 promotes neurite outgrowth through exocytic fusion of Rab11- and L1-containing vesicles by releasing exocyst component Exo70.PLoS One. 2013 Nov 4;8(11):e79689. doi: 10.1371/journal.pone.0079689. eCollection 2013. PLoS One. 2013. PMID: 24223996 Free PMC article.
-
The Exocyst at a Glance.J Cell Sci. 2015 Aug 15;128(16):2957-64. doi: 10.1242/jcs.156398. Epub 2015 Aug 3. J Cell Sci. 2015. PMID: 26240175 Free PMC article. Review.
-
The role of Exo70 in exocytosis and beyond.Small GTPases. 2019 Sep;10(5):331-335. doi: 10.1080/21541248.2017.1328998. Epub 2017 Jun 19. Small GTPases. 2019. PMID: 28489961 Free PMC article. Review.
Cited by
-
SNAP23/25 and VAMP2 mediate exocytic event of transferrin receptor-containing recycling vesicles.Biol Open. 2015 Jun 19;4(7):910-20. doi: 10.1242/bio.012146. Biol Open. 2015. PMID: 26092867 Free PMC article.
-
The temporally controlled expression of Drongo, the fruit fly homolog of AGFG1, is achieved in female germline cells via P-bodies and its localization requires functional Rab11.RNA Biol. 2016 Nov;13(11):1117-1132. doi: 10.1080/15476286.2016.1218592. Epub 2016 Aug 11. RNA Biol. 2016. PMID: 27654348 Free PMC article.
-
Bi-allelic VPS16 variants limit HOPS/CORVET levels and cause a mucopolysaccharidosis-like disease.EMBO Mol Med. 2021 May 7;13(5):e13376. doi: 10.15252/emmm.202013376. Epub 2021 May 3. EMBO Mol Med. 2021. PMID: 33938619 Free PMC article.
-
Host Cell Signatures of the Envelopment Site within Beta-Herpes Virions.Int J Mol Sci. 2022 Sep 1;23(17):9994. doi: 10.3390/ijms23179994. Int J Mol Sci. 2022. PMID: 36077391 Free PMC article. Review.
-
Rab-mediated vesicle trafficking in cancer.J Biomed Sci. 2016 Oct 6;23(1):70. doi: 10.1186/s12929-016-0287-7. J Biomed Sci. 2016. PMID: 27716280 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
