Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain

doi:10.1590/s0001-37652012000200018

. 2012 Jun;84(2):495-508.

doi: 10.1590/s0001-37652012000200018.

Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain

Elinalva M Paulo¹, Elisangela F Boffo, Alexsandro Branco, Angela M M P Valente, Itamar S Melo, Antonio G Ferreira, Milton R A Roque, Sandra A de Assis

Affiliations

PMID: 22652760
DOI: 10.1590/s0001-37652012000200018

Free article

Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain

Elinalva M Paulo et al. An Acad Bras Cienc. 2012 Jun.

Free article

. 2012 Jun;84(2):495-508.

doi: 10.1590/s0001-37652012000200018.

Authors

Elinalva M Paulo¹, Elisangela F Boffo, Alexsandro Branco, Angela M M P Valente, Itamar S Melo, Antonio G Ferreira, Milton R A Roque, Sandra A de Assis

Affiliation

¹ Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, BA, Brasil.

PMID: 22652760
DOI: 10.1590/s0001-37652012000200018

Abstract

The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28ºC for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and EPS II). The identification of EPS I and EPS II was conducted using FT-IR, (1)H, (13)C and DEPT-135 NMR spectra. The two substances were identified as linear dextran α polysaccharides (1 → 6) which indicated different characteristics with respect to thermal analysis and density of free packaging, viscosity and time of solubilization. Both dextrans are of low density, possess high thermal stability and exhibited the behavior characteristic of pseudoplastic polymers.

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