Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Dec;87(24):9958-61.
doi: 10.1073/pnas.87.24.9958.

Frequent mutation of the p53 gene in human esophageal cancer

M C Hollstein  1 , R A Metcalf, J A Welsh, R Montesano, C C Harris
Affiliations

Frequent mutation of the p53 gene in human esophageal cancer

M C Hollstein et al. Proc Natl Acad Sci U S A. 1990 Dec.

Abstract

Sequence alterations in the p53 gene have been detected in human tumors of the brain, breast, lung, and colon, and it has been proposed that p53 mutations spanning a major portion of the coding region inactivate the tumor suppressor function of this gene. To our knowledge, neither transforming mutations in oncogenes nor mutations in tumor suppressor genes have been reported in human esophageal tumors. We examined four human esophageal carcinoma cell lines and 14 human esophageal squamous cell carcinomas by polymerase chain reaction amplification and direct sequencing for the presence of p53 mutations in exons 5, 6, 7, 8, and 9. Two cell lines and five of the tumor specimens contained a mutated allele (one frameshift and six missense mutations). All missense mutations detected occurred at G.C base pairs in codons at or adjacent to mutations previously reported in other cancers. The identification of aberrant p53 gene alleles in one-third of the tumors we tested suggests that mutations at this locus are common genetic events in the pathogenesis of squamous cell carcinomas of the esophagus.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Cancer Res. 1988 Aug 1;48(15):4135-43 - PubMed
    1. Lancet. 1990 Mar 24;335(8691):675-9 - PubMed
    1. Mutat Res. 1989 Jul;213(1):3-25 - PubMed
    1. Environ Mol Mutagen. 1990;15(2):107-22 - PubMed
    1. Cancer Res. 1988 Sep 15;48(18):5119-23 - PubMed