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. 2012 Jun 12;586(12):1736-41.
doi: 10.1016/j.febslet.2012.05.012. Epub 2012 May 18.

Redesigning the single-chain variant of the restriction endonuclease PvuII by circular permutation

Benno Schierling  1 , Wolfgang Wende, Alfred Pingoud
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Free article

Redesigning the single-chain variant of the restriction endonuclease PvuII by circular permutation

Benno Schierling et al. FEBS Lett. .
Free article

Abstract

The restriction endonuclease PvuII has been introduced as a sequence-specific cleavage module in highly-specific nucleases for gene targeting. Here, a structural reorganization of the single-chain variant of PvuII (scPvuII) was performed by circular permutation as a proof-of-concept in order to find out whether the relocated, new termini next to structural elements important for DNA recognition and catalysis could be used for the fusion with other regulatory protein domains. Three circularly permuted variants of scPvuII were obtained that all maintain the specific endonucleolytic activity of scPvuII.

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