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. 2012;7(5):e36425.
doi: 10.1371/journal.pone.0036425. Epub 2012 May 7.

Hepcidin expression in iron overload diseases is variably modulated by circulating factors

Giulia Ravasi  1 , Sara Pelucchi, Paola Trombini, Raffaella Mariani, Naohisa Tomosugi, Giulia Litta Modignani, Matteo Pozzi, Elizabeth Nemeth, Tomas Ganz, Hisao Hayashi, Donatella Barisani, Alberto Piperno
Affiliations

Hepcidin expression in iron overload diseases is variably modulated by circulating factors

Giulia Ravasi et al. PLoS One. 2012.

Abstract

Hepcidin is a regulatory hormone that plays a major role in controlling body iron homeostasis. Circulating factors (holotransferrin, cytokines, erythroid regulators) might variably contribute to hepcidin modulation in different pathological conditions. There are few studies analysing the relationship between hepcidin transcript and related protein expression profiles in humans. Our aims were: a. to measure hepcidin expression at either hepatic, serum and urinary level in three paradigmatic iron overload conditions (hemochromatosis, thalassemia and dysmetabolic iron overload syndrome) and in controls; b. to measure mRNA hepcidin expression in two different hepatic cell lines (HepG2 and Huh-7) exposed to patients and controls sera to assess whether circulating factors could influence hepcidin transcription in different pathological conditions. Our findings suggest that hepcidin assays reflect hepatic hepcidin production, but also indicate that correlation is not ideal, likely due to methodological limits and to several post-trascriptional events. In vitro study showed that THAL sera down-regulated, HFE-HH and C-NAFLD sera up-regulated hepcidin synthesis. HAMP mRNA expression in Huh-7 cells exposed to sera form C-Donors, HFE-HH and THAL reproduced, at lower level, the results observed in HepG2, suggesting the important but not critical role of HFE in hepcidin regulation.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

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