Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Apr;30(1):218-24.
doi: 10.1128/JVI.30.1.218-224.1979.

Histone modifications in simian virus 40 and in nucleoprotein complexes containing supercoiled viral DNA

Y H Chen, J P MacGregor, D A Goldstein, M R Hall

Histone modifications in simian virus 40 and in nucleoprotein complexes containing supercoiled viral DNA

Y H Chen et al. J Virol. 1979 Apr.

Abstract

Simian virus (SV40) nucleoprotein complexes containing circular supercoiled viral DNA were extracted from infected cells and purified by differential centrifugation. The protein content of these complexes was compared by electrophoresis on 15% acrylamide gels with the protein content of purified SV40 virions and with histones from virus-infected cells. The electrophoretic patterns of histones from each of the sources revealed several major differences. SV40 virions contained histones H3, H2B, H2A, and H4 but not H1. Nucleoprotein complexes and host cells contained all five major histone groups. Relative to cellular histones, virion and nucleoprotein complex histones were enriched 15 to 40% in histones H3 and H4. In addition to the major classes of histones, several subfractions of histones H1, H3, and H4 were observed in acrylamide gels of proteins from SV40 virions and viral nucleoprotein complexes. Acetate labeling experiments indicated that each subfraction of histones H3 and H4 had a different level of acetylation. The histones from SV40 virions and nucleoprotein complexes were acetylated to significantly higher levels than those of infected host cells. No apparent differences in phosphorylation of the major histone groups were observed.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Virology. 1959 Nov;9:488-9 - PubMed
    1. J Mol Biol. 1965 Feb;11:149-65 - PubMed
    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Nucleic Acids Res. 1977 Dec;4(12):4261-77 - PubMed
    1. Cell. 1977 Jul;11(3):495-504 - PubMed

Publication types

LinkOut - more resources