Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Nov;87(22):8835-9.
doi: 10.1073/pnas.87.22.8835.

Molecular cloning of the large subunit of transforming growth factor type beta masking protein and expression of the mRNA in various rat tissues

T Tsuji  1 , F Okada, K Yamaguchi, T Nakamura
Affiliations

Molecular cloning of the large subunit of transforming growth factor type beta masking protein and expression of the mRNA in various rat tissues

T Tsuji et al. Proc Natl Acad Sci U S A. 1990 Nov.

Abstract

Masking protein (MP), which neutralizes the activity of transforming growth factor type beta 1 (TGF-beta 1), is composed of a dimeric N-terminal part of a TGF-beta 1 precursor of Mr 39,000 and an unknown large subunit of Mr 105,000-120,000. The deduced primary structure of the MP large subunit was elucidated by determining the nucleotide sequence of its cDNA. The cDNA encodes a prepro-precursor of 1712 amino acid residues with a calculated Mr of 186,596. The mature large subunit seems to be derived proteolytically from a prepro-precursor and the calculated Mr is 91,606. The precursor has seven N-linked glycosylation sites and an unusual structure containing 18 epidermal growth factor-like domains and four cysteine-rich internal repeats. The large subunit mRNA is synthesized in parallel with the expression of TGF-beta 1 mRNA in various rat tissues.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Cell. 1990 Jun 15;61(6):1051-61 - PubMed
    1. J Mol Biol. 1982 May 5;157(1):105-32 - PubMed
    1. Eur J Biochem. 1983 Jun 1;133(1):17-21 - PubMed
    1. J Lab Clin Med. 1984 May;103(5):811-8 - PubMed

Publication types

Associated data

LinkOut - more resources