Microbial community succession on developing lesions on human enamel
- PMID: 22432048
- PMCID: PMC3307376
- DOI: 10.3402/jom.v4i0.16125
Microbial community succession on developing lesions on human enamel
Abstract
Background: Dental caries is one of the most common diseases in the world. However, our understanding of how the microbial community composition changes in vivo as caries develops is lacking.
Objective: An in vivo model was used in a longitudinal cohort study to investigate shifts in the microbial community composition associated with the development of enamel caries.
Design: White spot lesions were generated in vivo on human teeth predetermined to be extracted for orthodontic reasons. The bacterial microbiota on sound enamel and on developing carious lesions were identified using the Human Oral Microbe Identification Microarray (HOMIM), which permits the detection of about 300 of the approximate 600 predominant bacterial species in the oral cavity.
Results: After only seven weeks, 75% of targeted teeth developed white spot lesions (8 individuals, 16 teeth). The microbial community composition of the plaque over white spot lesions differed significantly as compared to sound enamel. Twenty-five bacterial taxa, including Streptococcusmutans, Atopobiumparvulum, Dialisterinvisus, and species of Prevotella and Scardovia, were significantly associated with initial enamel lesions. In contrast, 14 bacterial taxa, including species of Fusobacterium, Campylobacter, Kingella, and Capnocytophaga, were significantly associated with sound enamel.
Conclusions: The bacterial community composition associated with the progression of enamel lesions is specific and much more complex than previously believed. This investigation represents one of the first longitudinally-derived studies for caries progression and supports microbial data from previous cross-sectional studies on the development of the disease. Thus, the in vivo experiments of generating lesions on teeth destined for extraction in conjunction with HOMIM analyses represent a valid model to study succession of supragingival microbial communities associated with caries development and to study efficacy of prophylactic and restorative treatments.
Keywords: HOMIM; caries; molecular microbiology; white spot lesions.
Figures
Similar articles
-
Microbiomes of Site-Specific Dental Plaques from Children with Different Caries Status.Infect Immun. 2017 Jul 19;85(8):e00106-17. doi: 10.1128/IAI.00106-17. Print 2017 Aug. Infect Immun. 2017. PMID: 28507066 Free PMC article.
-
Human root caries: microbiota in plaque covering sound, carious and arrested carious root surfaces.Caries Res. 1995;29(5):382-95. doi: 10.1159/000262097. Caries Res. 1995. PMID: 8521441
-
Bacteriologic status of non-cavitated proximal enamel caries lesions. A histologic and histobacteriologic study.J Dent. 2020 Sep;100:103422. doi: 10.1016/j.jdent.2020.103422. Epub 2020 Jun 29. J Dent. 2020. PMID: 32615236
-
Durability of esthetic improvement following Icon resin infiltration of multibracket-induced white spot lesions compared with no therapy over 6 months: a single-center, split-mouth, randomized clinical trial.Am J Orthod Dentofacial Orthop. 2013 Jul;144(1):86-96. doi: 10.1016/j.ajodo.2013.02.029. Am J Orthod Dentofacial Orthop. 2013. PMID: 23810050 Clinical Trial.
-
The in vivo orthodontic banding model for vital teeth and the in situ orthodontic banding model for hard-tissue slabs.J Dent Res. 1992 Apr;71 Spec No:832-5. doi: 10.1177/002203459207100S08. J Dent Res. 1992. PMID: 1592969 Review.
Cited by
-
Caries Etiology and Preventive Measures.Eur J Dent. 2024 Jul;18(3):766-776. doi: 10.1055/s-0043-1777051. Epub 2024 Mar 31. Eur J Dent. 2024. PMID: 38555649 Free PMC article.
-
The Evolving Microbiome of Dental Caries.Microorganisms. 2024 Jan 7;12(1):121. doi: 10.3390/microorganisms12010121. Microorganisms. 2024. PMID: 38257948 Free PMC article. Review.
-
Dental caries and their microbiomes in children: what do we do now?J Oral Microbiol. 2023 Apr 10;15(1):2198433. doi: 10.1080/20002297.2023.2198433. eCollection 2023. J Oral Microbiol. 2023. PMID: 37056224 Free PMC article. Review.
-
Identification of a novel bacterium Scardovia wiggsiae in high caries risk adolescence: A metagenomic and melt curve analysis.J Conserv Dent. 2022 May-Jun;25(3):297-305. doi: 10.4103/jcd.jcd_79_22. Epub 2022 Jun 13. J Conserv Dent. 2022. PMID: 35836558 Free PMC article.
-
Association between Bifidobacterium and Scardovia Wiggsiae and caries-related factors in severe early childhood caries and caries-free Thai children: a quantitative real-time PCR analysis and a questionnaire cross-sectional study.Eur Arch Paediatr Dent. 2022 Jun;23(3):437-447. doi: 10.1007/s40368-022-00702-0. Epub 2022 Apr 7. Eur Arch Paediatr Dent. 2022. PMID: 35389204
References
-
- van Houte J. Role of micro-organisms in caries etiology. J Dent Res. 1994;73:672–81. - PubMed
-
- Kleinberg I. A mixed-bacteria ecological approach to understanding the role of the oral bacteria in dental caries causation: an alternative to Streptococcus mutans and the specific-plaque hypothesis. Crit Rev Oral Biol Med. 2002;13:108–25. - PubMed
-
- Marsh PD. Are dental diseases examples of ecological catastrophes? Microbiology. 2003;149:279–94. - PubMed
-
- Beighton D. The complex oral microflora of high-risk individuals and groups and its role in the caries process. Community Dent Oral Epidemiol. 2005;33:248–55. - PubMed
-
- Takahashi N, Nyvad B. Caries ecology revisited: microbial dynamics and the caries process. Caries Res. 2008;42:409–18. - PubMed
LinkOut - more resources
Full Text Sources