Surface modified magnetic nanoparticles for immuno-gene therapy of murine mammary adenocarcinoma
- PMID: 22429983
- DOI: 10.1016/j.biomaterials.2012.02.061
Surface modified magnetic nanoparticles for immuno-gene therapy of murine mammary adenocarcinoma
Abstract
Cancer immuno-gene therapy is an introduction of nucleic acids encoding immunostimulatory proteins, such as cytokine interleukin 12 (IL-12), into somatic cells to stimulate an immune response against a tumor. Various methods can be used for the introduction of nucleic acids into cells; magnetofection involves binding of nucleic acids to magnetic nanoparticles with subsequent exposure to an external magnetic field. Here we show that surface modified superparamagnetic iron oxide nanoparticles (SPIONs) with a combination of polyacrylic acid (PAA) and polyethylenimine (PEI) (SPIONs-PAA-PEI) proved to be safe and effective for magnetofection of cells and tumors in mice. Magnetofection of cells with plasmid DNA encoding reporter gene using SPIONs-PAA-PEI was superior in transfection efficiency to commercially available SPIONs. Magnetofection of murine mammary adenocarcinoma with plasmid DNA encoding IL-12 using SPIONs-PAA-PEI resulted in significant antitumor effect and could be further refined for cancer immuno-gene therapy.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Similar articles
-
Magnetic field contributes to the cellular uptake for effective therapy with magnetofection using plasmid DNA encoding against Mcam in B16F10 melanoma in vivo.Nanomedicine (Lond). 2016 Mar;11(6):627-41. doi: 10.2217/nnm.16.4. Epub 2016 Mar 8. Nanomedicine (Lond). 2016. PMID: 27021639
-
Chondroitin sulfate-polyethylenimine copolymer-coated superparamagnetic iron oxide nanoparticles as an efficient magneto-gene carrier for microRNA-encoding plasmid DNA delivery.Nanoscale. 2015 May 14;7(18):8554-65. doi: 10.1039/c5nr01404b. Nanoscale. 2015. PMID: 25897645
-
Tumor-targeting magnetic lipoplex delivery of short hairpin RNA suppresses IGF-1R overexpression of lung adenocarcinoma A549 cells in vitro and in vivo.Biochem Biophys Res Commun. 2011 Jul 8;410(3):537-42. doi: 10.1016/j.bbrc.2011.06.019. Epub 2011 Jun 12. Biochem Biophys Res Commun. 2011. PMID: 21683689
-
Magnetically enhanced nucleic acid delivery. Ten years of magnetofection-progress and prospects.Adv Drug Deliv Rev. 2011 Nov;63(14-15):1300-31. doi: 10.1016/j.addr.2011.08.002. Epub 2011 Aug 26. Adv Drug Deliv Rev. 2011. PMID: 21893135 Free PMC article. Review.
-
Magnetic field-assisted gene delivery: achievements and therapeutic potential.Curr Gene Ther. 2012 Apr 1;12(2):116-26. doi: 10.2174/156652312800099616. Curr Gene Ther. 2012. PMID: 22348552 Review.
Cited by
-
Magnetic Hyperthermia for Cancer Treatment: Main Parameters Affecting the Outcome of In Vitro and In Vivo Studies.Molecules. 2020 Jun 22;25(12):2874. doi: 10.3390/molecules25122874. Molecules. 2020. PMID: 32580417 Free PMC article. Review.
-
Alginate-Based Platforms for Cancer-Targeted Drug Delivery.Biomed Res Int. 2020 Oct 7;2020:1487259. doi: 10.1155/2020/1487259. eCollection 2020. Biomed Res Int. 2020. PMID: 33083451 Free PMC article. Review.
-
Delivery of drugs, proteins, and nucleic acids using inorganic nanoparticles.Adv Drug Deliv Rev. 2020;156:188-213. doi: 10.1016/j.addr.2020.06.020. Epub 2020 Jun 29. Adv Drug Deliv Rev. 2020. PMID: 32610061 Free PMC article. Review.
-
Visualization of internalization of functionalized cobalt ferrite nanoparticles and their intracellular fate.Int J Nanomedicine. 2013;8:919-31. doi: 10.2147/IJN.S38749. Epub 2013 Mar 3. Int J Nanomedicine. 2013. PMID: 23486857 Free PMC article.
-
Cardiac-targeting magnetic lipoplex delivery of SH-IGF1R plasmid attenuate norepinephrine-induced cardiac hypertrophy in murine heart.Biosci Rep. 2014 Oct 2;34(5):e00140. doi: 10.1042/BSR20130107. Biosci Rep. 2014. PMID: 24965872 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
