Diabetes-impaired wound healing and altered macrophage activation: a possible pathophysiologic correlation
- PMID: 22380690
- DOI: 10.1111/j.1524-475X.2012.00772.x
Diabetes-impaired wound healing and altered macrophage activation: a possible pathophysiologic correlation
Abstract
Macrophages play a critical role in wound healing and can be activated to two distinctive phenotypes in vitro: classical macrophage activation (caM) and alternative macrophage activation (aaM). This study investigated whether the impaired cutaneous repair observed in streptozotocin-induced diabetic rats was associated with altered macrophage activation. Our results show that macrophage activation phenotypes could be observed in wound healing through double immunostaining. The caM macrophages appeared in the initial stage of wound healing, followed by aaM macrophages, which predominated in normal wounds. However, through examining markers associated with activation by immunoblotting and real-time polymerase chain reaction (PCR), diabetic wounds demonstrated insufficient caM in the early stage but excessive aaM in the later proliferative phase. Moreover, the macrophage activation markers were correlated with the instructive T helper cell type 1 (Th1)/Th2 cytokines in both groups. It was indicated that changed macrophage activation might contribute to impaired healing in diabetes wounds, and that strategies for reverting this abnormal activation could be useful for enhancing the wound healing process.
© 2012 by the Wound Healing Society.
Similar articles
-
Glucocorticoid-dependent impairment of wound healing in experimental diabetes: amelioration by adrenalectomy and RU 486.J Surg Res. 1999 Apr;82(2):234-43. doi: 10.1006/jsre.1998.5541. J Surg Res. 1999. PMID: 10090835
-
Pseudomonas aeruginosa infection alters the macrophage phenotype switching process during wound healing in diabetic mice.Cell Biol Int. 2018 Jul;42(7):877-889. doi: 10.1002/cbin.10955. Epub 2018 Mar 14. Cell Biol Int. 2018. PMID: 29512223
-
Effects of plasma fibronectin on the healing of full-thickness skin wounds in streptozotocin-induced diabetic rats.J Surg Res. 2007 Mar;138(1):64-70. doi: 10.1016/j.jss.2006.06.034. Epub 2006 Dec 11. J Surg Res. 2007. PMID: 17161431
-
Macrophages in skin injury and repair.Immunobiology. 2011 Jul;216(7):753-62. doi: 10.1016/j.imbio.2011.01.001. Epub 2011 Jan 8. Immunobiology. 2011. PMID: 21281986 Review.
-
Macrophages - sensors and effectors coordinating skin damage and repair.J Dtsch Dermatol Ges. 2014 Mar;12(3):214-21, 214-23. doi: 10.1111/ddg.12290. J Dtsch Dermatol Ges. 2014. PMID: 24580874 Review.
Cited by
-
Inflammation-Modulating Biomedical Interventions for Diabetic Wound Healing: An Overview of Preclinical and Clinical Studies.ACS Omega. 2024 Nov 1;9(45):44860-44875. doi: 10.1021/acsomega.4c02251. eCollection 2024 Nov 12. ACS Omega. 2024. PMID: 39554458 Free PMC article. Review.
-
Potential Role of CXCL10 in Monitoring Response to Treatment in Leprosy Patients.Front Immunol. 2021 Jul 20;12:662307. doi: 10.3389/fimmu.2021.662307. eCollection 2021. Front Immunol. 2021. PMID: 34354699 Free PMC article.
-
Hypoxic microenvironment promotes diabetic wound healing by polarizing macrophages to the M2 phenotype in vivo.J Mol Histol. 2024 Oct;55(5):967-976. doi: 10.1007/s10735-024-10244-y. Epub 2024 Aug 10. J Mol Histol. 2024. PMID: 39122894
-
Cellular Therapeutics for Chronic Wound Healing: Future for Regenerative Medicine.Curr Drug Targets. 2022;23(16):1489-1504. doi: 10.2174/138945012309220623144620. Curr Drug Targets. 2022. PMID: 35748548 Review.
-
The physiological phenomenon and regulation of macrophage polarization in diabetic wound.Mol Biol Rep. 2023 Nov;50(11):9469-9477. doi: 10.1007/s11033-023-08782-x. Epub 2023 Sep 9. Mol Biol Rep. 2023. PMID: 37688679 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
