Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Feb 26;14(4):343-54.
doi: 10.1038/ncb2445.

A 14-3-3γ dimer-based scaffold bridges CtBP1-S/BARS to PI(4)KIIIβ to regulate post-Golgi carrier formation

Carmen Valente  1 , Gabriele Turacchio, Stefania Mariggiò, Alessandro Pagliuso, Renato Gaibisso, Giuseppe Di Tullio, Michele Santoro, Fabio Formiggini, Stefania Spanò, Daniele Piccini, Roman S Polishchuk, Antonino Colanzi, Alberto Luini, Daniela Corda
Affiliations

A 14-3-3γ dimer-based scaffold bridges CtBP1-S/BARS to PI(4)KIIIβ to regulate post-Golgi carrier formation

Carmen Valente et al. Nat Cell Biol. .

Abstract

Large pleiomorphic carriers leave the Golgi complex for the plasma membrane by en bloc extrusion of specialized tubular domains, which then undergo fission. Several components of the underlying molecular machinery have been identified, including those involved in the budding/initiation of tubular carrier precursors (for example, the phosphoinositide kinase PI(4)KIIIβ, the GTPase ARF, and FAPP2), and in the fission of these precursors (for example, PKD, CtBP1-S/BARS). However, how these proteins interact to bring about carrier formation is poorly understood. Here, we describe a protein complex that mediates carrier formation and contains budding and fission molecules, as well as other molecules, such as the adaptor protein 14-3-3γ. Specifically, we show that 14-3-3γ dimers bridge CtBP1-S/BARS with PI(4)KIIIβ, and that the resulting complex is stabilized by phosphorylation by PKD and PAK. Disrupting the association of these proteins inhibits the fission of elongating carrier precursors, indicating that this complex couples the carrier budding and fission processes.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Biol Chem. 2008 Oct 10;283(41):27717-27723 - PubMed
    1. Biochem J. 2008 Sep 1;414(2):189-94 - PubMed
    1. EMBO J. 2003 Jun 16;22(12):3122-30 - PubMed
    1. Nature. 2005 Dec 1;438(7068):675-8 - PubMed
    1. J Biol Chem. 2002 Jan 18;277(3):2012-8 - PubMed

Publication types

LinkOut - more resources