Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Jun 25;6(8):2879-99.
doi: 10.1093/nar/6.8.2879.

Identification and mapping of N6-methyladenosine containing sequences in simian virus 40 RNA

D Canaani, C Kahana, S Lavi, Y Groner
Free PMC article

Identification and mapping of N6-methyladenosine containing sequences in simian virus 40 RNA

D Canaani et al. Nucleic Acids Res. .
Free PMC article

Abstract

Late SV40 16S and 19S mRNAs were found to contain an average of three m6A residues per mRNA molecule. The methylated residues of both the viral and cellular mRNAs occur in two sequences; Gpm6ApC and (Ap)nm6ApC, where n = 1-4. More than 60% of the m6A residues in SV40 16S and 19S mRNAs occur in Gpm6ApC even though there are twice as many (A)nAC than GAC sequences in these messengers. The m6A containing oligonucleotides of late SV40 MRNAs were localized in the viral messengers. In the 16S mRNA two m6A oligonucleotides were located at the 5' coding region between 0.95--0.0 map units. The third m6A residue was mapped between 0.0--0.14 map units in the translated portion of this mRNA. The overall pattern of internal methylation in the 19S mRNA is similar. However, some differences between 16S and 19S mRNAs were observed in both the content and location of the longer (Ap)n m6AC nucleotides. These results provide the first example of precise localization of internal methylation sequences in mRNA species with defined coding specificity. It implies that a) location of m6A residues is not random but specific to a particular region of the RNA, b) apart from sequence specificity other structural features of the mRNA may influence internal methylation and c) m6A residues are present in coding regions of SV40 mRNAs.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nat New Biol. 1973 May 2;243(122):2-6 - PubMed
    1. J Mol Biol. 1964 Nov;10:353-6 - PubMed
    1. Annu Rev Biochem. 1978;47:1079-126 - PubMed
    1. J Mol Biol. 1977 Oct 5;115(4):695-714 - PubMed
    1. Biochemistry. 1978 Mar 21;17(6):977-82 - PubMed

Publication types