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. 2012 Apr;78(8):2988-90.
doi: 10.1128/AEM.07093-11. Epub 2012 Feb 3.

Acquired tolerance to oxidative stress in Bifidobacterium longum 105-A via expression of a catalase gene

Jianlong He  1 , Kouta Sakaguchi, Tohru Suzuki
Affiliations

Acquired tolerance to oxidative stress in Bifidobacterium longum 105-A via expression of a catalase gene

Jianlong He et al. Appl Environ Microbiol. 2012 Apr.

Abstract

For improvement of tolerance to oxidative stress in Bifidobacterium longum 105-A, we introduced the Bacillus subtilis catalase gene (katE) into it. The transformant showed catalase activity (39 U/mg crude protein) in the intracellular fraction, which increased survival by ∼100-fold after a 1-h exposure to 4.4 mM H(2)O(2), decreased de novo H(2)O(2) accumulation, and increased survival in aerated cultures by 10(5)-fold at 24 h. The protection level was better than that conferred by exogenously added catalase.

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Figures

Fig 1
Fig 1
Cultured B. longum 105-A(pKKT427 or pBCAT001) under aerobic or anaerobic conditions. (A) Growth (OD660) of the cultured B. longum 105-A strain. (B) Counts of B. longum 105-A cultured under aerobic conditions. (C) LIVE/DEAD (L/D) assay of B. longum 105-A cultured under aerobic conditions. (D) H2O2 accumulation by B. longum 105-A cultured under aerobic conditions. Dotted line, B. longum 105-A(pKKT427); solid line, B. longum 105-A(pBCAT001); solid circles, B. longum 105-A(pKKT427) cultured under aerobic conditions; open circles, B. longum 105-A(pBCAT001) cultured under aerobic conditions; solid triangles, B. longum 105-A(pKKT427) cultured under anaerobic conditions; open triangles, B. longum 105-A(pBCAT001) cultured under anaerobic conditions; solid squares, B. longum 105-A(pKKT427) cultured using exogenously added catalase (3,000 U/ml medium) under aerobic conditions; open squares, B. longum 105-A(pKKT427) cultured using exogenously added catalase (100 U/ml medium) under aerobic conditions. The results presented correspond to the averages of three different assays. Error bars correspond to the standard errors of the mean values.
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