The hepatitis B virus-encoded transcriptional trans-activator hbx appears to be a novel protein serine/threonine kinase
- PMID: 2225072
- DOI: 10.1016/0092-8674(90)90135-2
The hepatitis B virus-encoded transcriptional trans-activator hbx appears to be a novel protein serine/threonine kinase
Erratum in
- Cell 1993 Nov 19;75(4):826
Abstract
To study the functional mechanism of the hepatitis B virus (HBV) X (hbx) gene product, we have expressed the hbx protein in E. coli and purified it by HPLC. The purified hbx protein was shown to be active in transactivating transcription directed by the LTR sequence of HIV-1. The hbx protein was found to have an intrinsic serine/threonine protein kinase activity. The hbx protein was detected in hepatitis B virions, and tryptic phosphopeptide maps of the hbx protein phosphorylated in the virion and of the in vitro phosphorylated bacterially expressed hbx protein were similar. Inactivation of the hbx protein by heat, protein-denaturing agents, or an ATP affinity analog, p-fluorosulfonylbenzoyl 5'-adenosine, resulted in loss of both protein kinase activity and trans-activation activity. These results suggest that the HBV-encoded trans-activator hbx is a novel protein kinase.
Similar articles
-
Expression and spectroscopic analysis of a mutant hepatitis B virus onco-protein HBx without cysteine residues.J Virol Methods. 2005 Jun;126(1-2):65-74. doi: 10.1016/j.jviromet.2005.01.022. J Virol Methods. 2005. PMID: 15847920
-
A transactivating function encoded in the hepatitis B virus X gene is conserved in the integrated state.Oncogene. 1988 Nov;3(5):545-52. Oncogene. 1988. PMID: 2856254
-
Hepatitis B virus X protein produced in Escherichia coli is biologically functional.J Virol. 1990 Aug;64(8):3963-6. doi: 10.1128/JVI.64.8.3963-3966.1990. J Virol. 1990. PMID: 2196386 Free PMC article.
-
Using proteomics to identify the HBx interactome in hepatitis B virus: how can this inform the clinic?Expert Rev Proteomics. 2014 Feb;11(1):59-74. doi: 10.1586/14789450.2014.861745. Epub 2013 Nov 29. Expert Rev Proteomics. 2014. PMID: 24308553 Review.
-
Hepatitis B Virus X and Regulation of Viral Gene Expression.Cold Spring Harb Perspect Med. 2016 Jan 8;6(3):a021402. doi: 10.1101/cshperspect.a021402. Cold Spring Harb Perspect Med. 2016. PMID: 26747833 Free PMC article. Review.
Cited by
-
Hepatitis B virus X antigen in the pathogenesis of chronic infections and the development of hepatocellular carcinoma.Am J Pathol. 1997 Apr;150(4):1141-57. Am J Pathol. 1997. PMID: 9094970 Free PMC article. Review.
-
Phosphorylation in the carboxyl-terminal domain of the capsid protein of hepatitis B virus: evaluation with a monoclonal antibody.J Virol. 1991 Nov;65(11):6024-30. doi: 10.1128/JVI.65.11.6024-6030.1991. J Virol. 1991. PMID: 1717713 Free PMC article.
-
Fas-activated serine/threonine kinase (FAST) phosphorylates TIA-1 during Fas-mediated apoptosis.J Exp Med. 1995 Sep 1;182(3):865-74. doi: 10.1084/jem.182.3.865. J Exp Med. 1995. PMID: 7544399 Free PMC article.
-
The herpes simplex virus 1 protein kinase encoded by the US3 gene mediates posttranslational modification of the phosphoprotein encoded by the UL34 gene.J Virol. 1991 Nov;65(11):5757-64. doi: 10.1128/JVI.65.11.5757-5764.1991. J Virol. 1991. PMID: 1656069 Free PMC article.
-
Induction of the DNA-binding activity of c-jun/c-fos heterodimers by the hepatitis B virus transactivator pX.Mol Cell Biol. 1994 Feb;14(2):989-98. doi: 10.1128/mcb.14.2.989-998.1994. Mol Cell Biol. 1994. PMID: 7507209 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
