Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Mar;19(3):396-400.
doi: 10.1128/CVI.05537-11. Epub 2012 Jan 11.

Development of a bead-based multiplex immunoassay for simultaneous quantitative detection of IgG serum antibodies against measles, mumps, rubella, and varicella-zoster virus

Gaby P Smits  1 , Pieter G van Gageldonk, Leo M Schouls, Fiona R M van der Klis, Guy A M Berbers
Affiliations

Development of a bead-based multiplex immunoassay for simultaneous quantitative detection of IgG serum antibodies against measles, mumps, rubella, and varicella-zoster virus

Gaby P Smits et al. Clin Vaccine Immunol. 2012 Mar.

Abstract

Enzyme-linked immunosorbent assay (ELISA) is normally used to quantify the amount of serum IgG antibodies against measles, mumps, rubella, and varicella-zoster virus (MMRV). However, this method is time- and material-consuming. Therefore, a multiplex immunoassay for the simultaneous quantitative detection of antibodies against MMRV was developed. In-house as well as commercially available antigens can be used, making the assay available for all laboratories. The multiplex assay is much more sensitive than the separate ELISAs and has a high specificity, and only 5 μl of serum is needed. Heterologous inhibition did not exceed 11.5%, while homologous inhibition varied between 91.3 and 97.9%. Good correlations with the in-house ELISAs for measles (R(2) = 0.98), mumps (R(2) = 0.97), and rubella (R(2) = 0.97) virus as well as with the ELISA kit for varicella-zoster virus (R(2) = 0.95) were obtained. In conclusion, the MMRV multiplex assay is a good alternative to the conventional ELISAs and suitable for use in serosurveillance and vaccine studies.

PubMed Disclaimer

Figures

Fig 1
Fig 1
Comparison of results obtained by the MIA with results from the in-house measles, mumps, and rubella virus ELISAs and the varicella-zoster virus ELISA kit. Cutoff levels are indicated by the horizontal and vertical dashed lines, and the ideal line is represented by the diagonal dashed line.
Fig 2
Fig 2
Comparison of results obtained by the MIA with results from measles and mumps virus ELISAs using commercially obtained antigens. Cutoff levels are indicated by the horizontal and vertical dashed lines, and the ideal line is represented by the diagonal dashed line.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Biagini RE, et al. 2003. Method for simultaneous measurement of antibodies to 23 pneumococcal capsular polysaccharides. Clin. Diagn. Lab. Immunol. 10: 744–750 - PMC - PubMed
    1. de Haas R, van den Hof S, Berbers GA, de Melker HE, Conyn-van Spaendonck MA. 1999. Prevalence of antibodies against rubella virus in The Netherlands 9 years after changing from selective to mass vaccination. Epidemiol. Infect. 123: 263–270 - PMC - PubMed
    1. De Melker HE, Conyn-van Spaendonck MA. 1998. Immunosurveillance and the evaluation of national immunization programmes: a population-based approach. Epidemiol. Infect. 121: 637–643 - PMC - PubMed
    1. de Voer RM, et al. 2008. Development of a fluorescent-bead-based multiplex immunoassay to determine immunoglobulin G subclass responses to Neisseria meningitidis serogroup A and C polysaccharides. Clin. Vaccine Immunol. 15: 1188–1193 - PMC - PubMed
    1. Dhiman N, et al. 2010. Detection of IgG-class antibodies to measles, mumps, rubella, and varicella-zoster virus using a multiplex bead immunoassay. Diagn. Microbiol. Infect. Dis. 67: 346–349 - PubMed

Publication types