Recent advances in genetic code engineering in Escherichia coli
- PMID: 22237016
- DOI: 10.1016/j.copbio.2011.12.027
Recent advances in genetic code engineering in Escherichia coli
Abstract
The expansion of the genetic code is gradually becoming a core discipline in Synthetic Biology. It offers the best possible platform for the transfer of numerous chemical reactions and processes from the chemical synthetic laboratory into the biochemistry of living cells. The incorporation of biologically occurring or chemically synthesized non-canonical amino acids into recombinant proteins and even proteomes via reprogrammed protein translation is in the heart of these efforts. Orthogonal pairs consisting of aminoacyl-tRNA synthetase and its cognate tRNA proved to be a general tool for the assignment of certain codons of the genetic code with a maximum degree of chemical liberty. Here, we highlight recent developments that should provide a solid basis for the development of generalist tools enabling a controlled variation of chemical composition in proteins and even proteomes. This will take place in the frame of a greatly expanded genetic code with emancipated codons liberated from the current function or with totally new coding units.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Similar articles
-
Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome.Nature. 2010 Mar 18;464(7287):441-4. doi: 10.1038/nature08817. Epub 2010 Feb 14. Nature. 2010. PMID: 20154731
-
Directed Evolution of the Methanosarcina barkeri Pyrrolysyl tRNA/aminoacyl tRNA Synthetase Pair for Rapid Evaluation of Sense Codon Reassignment Potential.Int J Mol Sci. 2021 Jan 18;22(2):895. doi: 10.3390/ijms22020895. Int J Mol Sci. 2021. PMID: 33477414 Free PMC article.
-
Engineering the Genetic Code in Cells and Animals: Biological Considerations and Impacts.Acc Chem Res. 2017 Nov 21;50(11):2767-2775. doi: 10.1021/acs.accounts.7b00376. Epub 2017 Oct 6. Acc Chem Res. 2017. PMID: 28984438 Free PMC article. Review.
-
tRNAPyl: Structure, function, and applications.RNA Biol. 2018;15(4-5):441-452. doi: 10.1080/15476286.2017.1356561. Epub 2017 Sep 13. RNA Biol. 2018. PMID: 28837402 Free PMC article. Review.
-
tRNA engineering for manipulating genetic code.RNA Biol. 2018;15(4-5):453-460. doi: 10.1080/15476286.2017.1343227. Epub 2017 Sep 6. RNA Biol. 2018. PMID: 28722545 Free PMC article. Review.
Cited by
-
Analysis of Stop Codons within Prokaryotic Protein-Coding Genes Suggests Frequent Readthrough Events.Int J Mol Sci. 2021 Feb 14;22(4):1876. doi: 10.3390/ijms22041876. Int J Mol Sci. 2021. PMID: 33672790 Free PMC article.
-
Synthetic Biological Circuits within an Orthogonal Central Dogma.Trends Biotechnol. 2021 Jan;39(1):59-71. doi: 10.1016/j.tibtech.2020.05.013. Epub 2020 Jun 22. Trends Biotechnol. 2021. PMID: 32586633 Free PMC article. Review.
-
Residue-Specific Incorporation of the Non-Canonical Amino Acid Norleucine Improves Lipase Activity on Synthetic Polyesters.Front Bioeng Biotechnol. 2022 Jan 26;10:769830. doi: 10.3389/fbioe.2022.769830. eCollection 2022. Front Bioeng Biotechnol. 2022. PMID: 35155387 Free PMC article.
-
Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation.Nucleic Acids Res. 2013 Jun;41(11):5949-63. doi: 10.1093/nar/gkt226. Epub 2013 Apr 15. Nucleic Acids Res. 2013. PMID: 23589624 Free PMC article.
-
Synthesis and Explosion Hazards of 4-Azido-l-phenylalanine.J Org Chem. 2018 Apr 20;83(8):4525-4536. doi: 10.1021/acs.joc.8b00270. Epub 2018 Apr 2. J Org Chem. 2018. PMID: 29577718 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
