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. 2012 Feb 15;28(4):593-4.
doi: 10.1093/bioinformatics/btr708. Epub 2011 Dec 23.

ART: a next-generation sequencing read simulator

Weichun Huang  1 , Leping Li, Jason R Myers, Gabor T Marth
Affiliations

ART: a next-generation sequencing read simulator

Weichun Huang et al. Bioinformatics. .

Abstract

ART is a set of simulation tools that generate synthetic next-generation sequencing reads. This functionality is essential for testing and benchmarking tools for next-generation sequencing data analysis including read alignment, de novo assembly and genetic variation discovery. ART generates simulated sequencing reads by emulating the sequencing process with built-in, technology-specific read error models and base quality value profiles parameterized empirically in large sequencing datasets. We currently support all three major commercial next-generation sequencing platforms: Roche's 454, Illumina's Solexa and Applied Biosystems' SOLiD. ART also allows the flexibility to use customized read error model parameters and quality profiles.

Availability: Both source and binary software packages are available at http://www.niehs.nih.gov/research/resources/software/art.

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