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. 2012 Mar;27(3):734-40.
doi: 10.3892/or.2011.1582. Epub 2011 Dec 7.

In vitro anti-neuroblastoma activity of saquinavir and its association with imatinib

Fabio Timeus  1 , Nicoletta Crescenzio, Alessandra Doria, Luiselda Foglia, Sara Pagliano, Emanuela Ricotti, Franca Fagioli, Pier Angelo Tovo, Luca Cordero di Montezemolo
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Free article

In vitro anti-neuroblastoma activity of saquinavir and its association with imatinib

Fabio Timeus et al. Oncol Rep. 2012 Mar.
Free article

Abstract

Neuroblastoma (NB) has a poor prognosis when in advanced stages, highlighting the need for new therapeutic options. The human immunodeficiency virus (HIV) protease inhibitor saquinavir is active in vitro against chronic myeloid leukaemia cells, in synergy with the tyrosine kinase inhibitor imatinib. Here, we evaluated the effects of saquinavir, alone or in association with imatinib, on cell proliferation (count of viable cells after trypan blue exclusion), apoptosis (Annexin V binding) and invasion (through a transwell membrane coated with Matrigel) in SJ-N-KP, IMR5, AF-8, SK-N-SH and SK-N-BE NB lines, all expressing c-kit and PDGF-R (determined by flow cytometry). Saquinavir showed a dose-dependent anti-proliferative and anti-invasive activity on NB lines, increased by the association with imatinib when the two drugs were utilized at clinically attainable concentrations. The same low saquinavir concentrations inhibited in NB cells the nuclear activation of NF-κB (Western immuno-blotting for nuclear NF-κB p50 and p65). Saquinavir at high concentrations also exerted a pro-apoptotic activity on NB lines, significantly increased by the association with imatinib. In conclusion, saquinavir and imatinib are both drugs utilized for long-term therapies, with good oral bioavailability and a well-known toxicity profile. The anti-NB activity of saquinavir and of its association with imatinib suggests a potential usefulness in the treatment of NB, particularly for remission maintenance.

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