Controlled gene expression in primary Lgr5 organoid cultures

doi:10.1038/nmeth.1802

. 2011 Dec 4;9(1):81-3.

doi: 10.1038/nmeth.1802.

Controlled gene expression in primary Lgr5 organoid cultures

Bon-Kyoung Koo¹, Daniel E Stange, Toshiro Sato, Wouter Karthaus, Henner F Farin, Meritxell Huch, Johan H van Es, Hans Clevers

Affiliations

PMID: 22138822
DOI: 10.1038/nmeth.1802

Controlled gene expression in primary Lgr5 organoid cultures

Bon-Kyoung Koo et al. Nat Methods. 2011.

. 2011 Dec 4;9(1):81-3.

doi: 10.1038/nmeth.1802.

Authors

Bon-Kyoung Koo¹, Daniel E Stange, Toshiro Sato, Wouter Karthaus, Henner F Farin, Meritxell Huch, Johan H van Es, Hans Clevers

Affiliation

¹ Hubrecht Institute for Developmental Biology and Stem Cell Research, and University Medical Centre, Utrecht, The Netherlands.

PMID: 22138822
DOI: 10.1038/nmeth.1802

Abstract

The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinase-inducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.

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References

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[1] Nature. 2009 May 14;459(7244):262-5 - PubMed

[2] Nature. 2009 May 14;459(7244):262-5 - PubMed

[3] Nature. 2011 Jul 04;476(7360):293-7 - PubMed

[4] Nature. 2011 Jul 04;476(7360):293-7 - PubMed

[5] Mol Cell Biol. 2003 Jan;23(2):655-64 - PubMed

[6] Mol Cell Biol. 2003 Jan;23(2):655-64 - PubMed

[7] Dev Biol. 2010 Oct 15;346(2):215-23 - PubMed

[8] Dev Biol. 2010 Oct 15;346(2):215-23 - PubMed

[9] J Biomed Biotechnol. 2009;2009:901079 - PubMed

[10] J Biomed Biotechnol. 2009;2009:901079 - PubMed

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Controlled gene expression in primary Lgr5 organoid cultures

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Controlled gene expression in primary Lgr5 organoid cultures

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