Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Feb;113(2):146-53.
doi: 10.1016/j.jbiosc.2011.10.006. Epub 2011 Nov 11.

Chicken oviduct-specific expression of transgene by a hybrid ovalbumin enhancer and the Tet expression system

Daisuke Kodama  1 , Daisuke Nishimiya, Ken-Ichi Nishijima, Yuuki Okino, Yujin Inayoshi, Yasuhiro Kojima, Ken-Ichiro Ono, Makoto Motono, Katsuhide Miyake, Yoshinori Kawabe, Kenji Kyogoku, Takashi Yamashita, Masamichi Kamihira, Shinji Iijima
Affiliations

Chicken oviduct-specific expression of transgene by a hybrid ovalbumin enhancer and the Tet expression system

Daisuke Kodama et al. J Biosci Bioeng. 2012 Feb.

Abstract

We generated genetically manipulated chickens and quail by infecting them with a retroviral vector expressing the human growth hormone under the control of chicken ovalbumin promoter/enhancer up to -3861 bp from the transcriptional start site. The growth hormone was expressed in an oviduct-specific manner and was found in egg white, although its level was low. The DNA sequence of the integrated form of the viral vector in the packaging cells was shown to be truncated and contained only the sequence spanning -3861 to -1569 bp. This represented only the DNase I hypersensitive site (DHS) III of the 4 DHSs and lacked the proximal promoter of the ovalbumin control region. We found several TATA-like and other promoter motifs of approximately -1800 bp and considered that these promoter motifs and DHS III may cause weak but oviduct-specific expression of the growth hormone. To prove this hypothesis and apply this system to oviduct-specific expression of the transgene, the truncated regulatory sequence was fused to an artificial transactivator-promoter system. In this system, initial weak but oviduct-specific expression of the Tet activator from the promoter element in the ovalbumin control sequence triggered a self-amplifying cycle of expression. DsRed was specifically expressed in oviduct cells of genetically manipulated chickens using this system. Furthermore, deletion of a short region possibly containing the promoter elements (-2112 to -1569 bp) completely abrogated oviduct-specific expression. Taken together, these results suggest that weak expression of this putative promoter causes oviduct-specific expression of the transgene.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

Publication types

Substances

LinkOut - more resources