Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jun;110(6):1911-22.
doi: 10.1083/jcb.110.6.1911.

Detection of an intermediate compartment involved in transport of alpha-factor from the plasma membrane to the vacuole in yeast

B Singer  1 , H Riezman
Affiliations

Detection of an intermediate compartment involved in transport of alpha-factor from the plasma membrane to the vacuole in yeast

B Singer et al. J Cell Biol. 1990 Jun.

Abstract

alpha-Factor, one of the mating pheromones of Saccharomyces cerevisiae, binds specifically to a receptor on the plasma membrane of a cells, is internalized and delivered to the vacuole, where it is degraded. At 15 degrees C the rate of pheromone uptake is only slightly affected while delivery to the vacuole is markedly slowed down. A transport intermediate carrying alpha-factor to the vacuole can be reversibly trapped by treatment with the metabolic inhibitors, NaN3 and NaF. This intermediate(s) is distinct from the vacuole and the plasma membrane as judged by differential and density gradient centrifugation analysis. We present evidence that the alpha-factor is protected from protease digestion by a detergent-sensitive structure, suggesting that the pheromone resides within a vesicular compartment. We propose that this intermediate(s) represents an endocytic or prevacuolar compartment(s) involved in vesicular traffic from the plasma membrane to the vacuole.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Cell Biol. 1989 Mar;108(3):843-53 - PubMed
    1. J Cell Biol. 1976 Oct;71(1):159-71 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Cell. 1985 Dec;43(3 Pt 2):643-52 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Nov;85(21):7972-6 - PubMed

Publication types

MeSH terms