Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1990 Jun;10(6):2544-53.
doi: 10.1128/mcb.10.6.2544-2553.1990.

SNF6 encodes a nuclear protein that is required for expression of many genes in Saccharomyces cerevisiae

F Estruch  1 , M Carlson
Affiliations
Comparative Study

SNF6 encodes a nuclear protein that is required for expression of many genes in Saccharomyces cerevisiae

F Estruch et al. Mol Cell Biol. 1990 Jun.

Abstract

The SNF6 gene appears to affect transcription from a variety of promoters in Saccharomyces cerevisiae. The gene was cloned, and sequence analysis revealed two completely overlapping open reading frames of 996 and 1092 nucleotides on opposite strands. The SNF6 coding sequence was identified by selective mutagenesis. The predicted 37,604-dalton SNF6 protein is highly charged but overall neutral. A bifunctional SNF6-beta-galactosidase fusion protein was localized in the nucleus, as judged by immunofluorescence microscopy. The N terminus of SNF6 contains a sequence homologous to nuclear localization signals and was sufficient to direct beta-galactosidase to the nucleus. The 5' ends of the SNF6 RNA were heterogeneous and included ends mapping downstream from the first ATG codon. Construction of a frameshift mutation provided evidence that translational initiation at the second ATG yields a partially functional SNF6 product. Null mutations in SNF6 caused a wider range of pleiotropic defects than the previously isolated point mutation, including slow growth. Genetic and molecular evidence suggested that SNF6 is functionally related to the SNF2 and SNF5 genes. These genes may function together to affect transcription.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Science. 1986 Sep 12;233(4769):1175-80 - PubMed
    1. Genetics. 1986 Apr;112(4):741-53 - PubMed
    1. Mol Cell Biol. 1986 Nov;6(11):3643-51 - PubMed
    1. Gene. 1986;45(3):299-310 - PubMed
    1. Mol Cell Biol. 1987 Feb;7(2):672-8 - PubMed

Publication types

Associated data