E3 ubiquitin ligase Siah-1 facilitates poly-ubiquitylation and proteasomal degradation of the hepatitis B viral X protein
- PMID: 21878328
- DOI: 10.1016/j.febslet.2011.08.015
E3 ubiquitin ligase Siah-1 facilitates poly-ubiquitylation and proteasomal degradation of the hepatitis B viral X protein
Abstract
Hepatitis B viral X protein (HBx) is a multifunctional transactivator and implicated in hepatitis B virus (HBV) replication and hepatocarcinogenesis. HBx can be ubiquitinated and degraded through ubiquitin-proteasome pathway. However, the E3 ubiquitin ligase regulating HBx ubiquitin-dependent degradation is still unknown. In this study, we identified Siah-1 as a novel E3 ubiquitin ligase for HBx, which interacted with HBx and facilitated HBx poly-ubiquitylation and proteasomal degradation. Co-expression of Siah-1 attenuated the transcriptional transactivation of HBx on glucocorticoid response element (GRE), heat shock response element (HSE) and cAMP response element (CRE) signal pathways. Moreover, Siah-1 participated in p53-mediated HBx degradation. Therefore, Siah-1 may play important roles in ubiquitin-dependent degradation of HBx and may be involved in suppressing the progression of hepatocellular carcinoma (HCC).
Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Similar articles
-
Hepatitis B virus X protein activates E3 ubiquitin ligase Siah-1 to control virus propagation via a negative feedback loop.J Gen Virol. 2017 Jul;98(7):1774-1784. doi: 10.1099/jgv.0.000856. Epub 2017 Jul 17. J Gen Virol. 2017. PMID: 28714848
-
Proteasomal activator 28 gamma stabilizes hepatitis B virus X protein by competitively inhibiting the Siah-1-mediated proteasomal degradation.Biochem Biophys Res Commun. 2021 Nov 12;578:97-103. doi: 10.1016/j.bbrc.2021.09.028. Epub 2021 Sep 16. Biochem Biophys Res Commun. 2021. PMID: 34555669
-
Hepatitis B virus X protein differentially affects the ubiquitin-mediated proteasomal degradation of beta-catenin depending on the status of cellular p53.J Gen Virol. 2007 Aug;88(Pt 8):2144-2154. doi: 10.1099/vir.0.82836-0. J Gen Virol. 2007. PMID: 17622616
-
Hepatitis B virus HBx protein interactions with the ubiquitin proteasome system.Viruses. 2014 Nov 24;6(11):4683-702. doi: 10.3390/v6114683. Viruses. 2014. PMID: 25421893 Free PMC article. Review.
-
Hepatocarcinogenesis in viral Hepatitis B infection: the role of HBx and p53.Acta Med Indones. 2006 Jul-Sep;38(3):154-9. Acta Med Indones. 2006. PMID: 16953033 Review.
Cited by
-
SIAH1-mediated RPS3 ubiquitination contributes to chemosensitivity in epithelial ovarian cancer.Aging (Albany NY). 2022 Aug 8;14(15):6202-6226. doi: 10.18632/aging.204211. Epub 2022 Aug 8. Aging (Albany NY). 2022. PMID: 35951361 Free PMC article.
-
Reactive Oxygen Species Induction by Hepatitis B Virus: Implications for Viral Replication in p53-Positive Human Hepatoma Cells.Int J Mol Sci. 2024 Jun 15;25(12):6606. doi: 10.3390/ijms25126606. Int J Mol Sci. 2024. PMID: 38928309 Free PMC article.
-
Tumor Suppressor p53 Inhibits Hepatitis B Virus Replication by Downregulating HBx via E6AP-Mediated Proteasomal Degradation in Human Hepatocellular Carcinoma Cell Lines.Viruses. 2022 Oct 21;14(10):2313. doi: 10.3390/v14102313. Viruses. 2022. PMID: 36298868 Free PMC article.
-
The E3 Ubiquitin Ligase Siah-1 Suppresses Avian Reovirus Infection by Targeting p10 for Degradation.J Virol. 2018 Feb 26;92(6):e02101-17. doi: 10.1128/JVI.02101-17. Print 2018 Mar 15. J Virol. 2018. PMID: 29321312 Free PMC article.
-
All-trans retinoic acid downregulates HBx levels via E6-associated protein-mediated proteasomal degradation to suppress hepatitis B virus replication.PLoS One. 2024 Jun 11;19(6):e0305350. doi: 10.1371/journal.pone.0305350. eCollection 2024. PLoS One. 2024. PMID: 38861553 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
Miscellaneous
