Apicidin, a histone deaceylase inhibitor, induces both apoptosis and autophagy in human oral squamous carcinoma cells
- PMID: 21856210
- DOI: 10.1016/j.oraloncology.2011.07.027
Apicidin, a histone deaceylase inhibitor, induces both apoptosis and autophagy in human oral squamous carcinoma cells
Abstract
Apicidin acts as a potent histone deacetylases (HDAC) inhibitor and the precise mechanism for its anti-tumor activity in human oral squamous cell carcinoma (OSCC) cells has not been examined. The aim of this study was to evaluate the anti-tumor efficacy of apicidin through apoptosis and autophagy in OSCC cells. Cells were treated with apicidin and cell death was quantified. Cell cycle and apoptosis were measured using flow cytometry assay, immunoblot. Autophagy was characterized by the increase of LC3B-II and the formation of acidic vesicular organelles (AVOs). Apicidin significantly inhibited the proliferation of OSCC cells in a dose-dependent manner. Apicidin markedly up-regulated p21(WAF1) led to G2/M phase arrest. Apicidin significantly increased the number of apoptotic cells compared to untreated control. Apicidin induced not only apoptosis but also autophagy in OSCC cells. Apicidin dramatically increased the levels of LC3 type II expression, ATG5 protein expression and the accumulation of AVOs. Inhibition of autophagy enhanced apicidin-mediated cytotoxicity through an increase in apoptosis. These results suggest that apicidin exerts anti-tumor effects by inducing apoptosis and autophagy and provide novel evidence of apicidin-induced autophagy and autophagy inhibition enhances apicidin-mediated apoptosis in OSCC cells.
Copyright © 2011 Elsevier Ltd. All rights reserved.
Similar articles
-
Synthesized Pheophorbide a-mediated photodynamic therapy induced apoptosis and autophagy in human oral squamous carcinoma cells.J Oral Pathol Med. 2013 Jan;42(1):17-25. doi: 10.1111/j.1600-0714.2012.01187.x. Epub 2012 Jun 28. J Oral Pathol Med. 2013. PMID: 22742535
-
Mechanism of apicidin-induced cell cycle arrest and apoptosis in Ishikawa human endometrial cancer cells.Chem Biol Interact. 2009 May 15;179(2-3):169-77. doi: 10.1016/j.cbi.2008.11.011. Epub 2008 Nov 25. Chem Biol Interact. 2009. PMID: 19070610
-
Apicidin inhibits cell growth by downregulating IGF-1R in salivary mucoepidermoid carcinoma cells.Oncol Rep. 2015 Apr;33(4):1899-907. doi: 10.3892/or.2015.3776. Epub 2015 Feb 2. Oncol Rep. 2015. PMID: 25647264
-
Systemic cell-cycle suppression by Apicidin, a histone deacetylase inhibitor, in MDA-MB-435 cells.Int J Mol Med. 2009 Aug;24(2):205-26. doi: 10.3892/ijmm_00000224. Int J Mol Med. 2009. PMID: 19578794
-
Emerging Role of Autophagy in the Development and Progression of Oral Squamous Cell Carcinoma.Cancers (Basel). 2021 Dec 7;13(24):6152. doi: 10.3390/cancers13246152. Cancers (Basel). 2021. PMID: 34944772 Free PMC article. Review.
Cited by
-
Advances in the Histone Acetylation Modification in the Oral Squamous Cell Carcinoma.J Oncol. 2023 Feb 9;2023:4616682. doi: 10.1155/2023/4616682. eCollection 2023. J Oncol. 2023. PMID: 39282225 Free PMC article. Review.
-
Epigenetic Control of Autophagy in Cancer Cells: A Key Process for Cancer-Related Phenotypes.Cells. 2019 Dec 17;8(12):1656. doi: 10.3390/cells8121656. Cells. 2019. PMID: 31861179 Free PMC article. Review.
-
Advances in the study of posttranslational modifications of histones in head and neck squamous cell carcinoma.Clin Epigenetics. 2024 Nov 21;16(1):165. doi: 10.1186/s13148-024-01785-w. Clin Epigenetics. 2024. PMID: 39574168 Free PMC article. Review.
-
Autophagic action of new targeting agents in head and neck oncology.Cancer Biol Ther. 2012 Sep;13(11):978-91. doi: 10.4161/cbt.21079. Epub 2012 Jul 24. Cancer Biol Ther. 2012. PMID: 22825332 Free PMC article. Review.
-
Epigenetic Targeting of Autophagy via HDAC Inhibition in Tumor Cells: Role of p53.Int J Mol Sci. 2018 Dec 8;19(12):3952. doi: 10.3390/ijms19123952. Int J Mol Sci. 2018. PMID: 30544838 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical