Interleukin-1 stimulates and all-trans-retinoic acid inhibits collagenase gene expression through its 5' activator protein-1-binding site
- PMID: 2178224
- DOI: 10.1210/mend-4-7-973
Interleukin-1 stimulates and all-trans-retinoic acid inhibits collagenase gene expression through its 5' activator protein-1-binding site
Abstract
Collagenase production by synovial fibroblast-like cells (synoviocytes) plays a major role in cartilage and bone destruction in rheumatoid arthritis. Interleukin-1 (IL-1) increases collagenase secretion by elevating the steady state levels of collagenase mRNA in cultured rheumatoid synoviocytes, while all-trans-retinoic acid (RA) has the opposite effect. We have studied the regulation of collagenase gene transcription by IL-1 and RA in synoviocytes by transient transfection of plasmid constructs containing deletion mutants of the 5'-flanking region of the collagenase gene or the isolated phorbol ester-responsive element ligated to a chloramphenicol acetyltransferase reporter gene. We show that the phorbol ester-responsive element of the collagenase gene mediates both positive and negative regulatory effects, respectively, of IL-1 and RA on transcription. In addition, we show that IL-1 and 12-O-tetradecanoyl-phorbol-13-acetate transiently induce c-jun and c-fos expression and that retinoic acid inhibits IL-1 and 12-O-tetradecanoyl-phorbol-13-acetate induction of c-fos, but not c-jun. These results suggest that RA inhibits collagenase transcription at least in part through inhibition of c-fos.
Similar articles
-
Regulation of human synovial fibroblast collagenase messenger RNA by interleukin-1.Arthritis Rheum. 1989 Dec;32(12):1539-45. doi: 10.1002/anr.1780321207. Arthritis Rheum. 1989. PMID: 2557044
-
Regulation of collagenase gene expression by okadaic acid, an inhibitor of protein phosphatases.Cell Regul. 1990 Feb;1(3):269-78. doi: 10.1091/mbc.1.3.269. Cell Regul. 1990. PMID: 1966042 Free PMC article.
-
Adenovirus E1A represses protease gene expression and inhibits metastasis of human tumor cells.Oncogene. 1990 Jan;5(1):75-83. Oncogene. 1990. PMID: 2157183
-
Structure and expression of type IV collagenase genes.Cell Differ Dev. 1990 Dec 2;32(3):307-12. doi: 10.1016/0922-3371(90)90044-w. Cell Differ Dev. 1990. PMID: 1965956 Review. No abstract available.
-
Transforming growth factor-beta in rheumatoid arthritis.Ann N Y Acad Sci. 1990;593:197-207. doi: 10.1111/j.1749-6632.1990.tb16112.x. Ann N Y Acad Sci. 1990. PMID: 2165375 Review. No abstract available.
Cited by
-
Differential regulation of collagenase gene expression by retinoic acid receptors--alpha, beta and gamma.Nucleic Acids Res. 1992 Jun 25;20(12):3105-11. doi: 10.1093/nar/20.12.3105. Nucleic Acids Res. 1992. PMID: 1320254 Free PMC article.
-
Monocyte chemoattractant protein-1 (MCP-1) expression in human articular cartilage. Induction by peptide regulatory factors and differential effects of dexamethasone and retinoic acid.J Clin Invest. 1992 Aug;90(2):488-96. doi: 10.1172/JCI115885. J Clin Invest. 1992. PMID: 1365641 Free PMC article.
-
Interleukin 1 induction of the c-jun promoter.Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7054-8. doi: 10.1073/pnas.90.15.7054. Proc Natl Acad Sci U S A. 1993. PMID: 8346217 Free PMC article.
-
Nuclear hormone receptors inhibit matrix metalloproteinase (MMP) gene expression through diverse mechanisms.Gene Expr. 1996;6(4):197-207. Gene Expr. 1996. PMID: 9196075 Free PMC article. Review.
-
Retinoic acid repression of cell-specific helix-loop-helix-octamer activation of the calcitonin/calcitonin gene-related peptide enhancer.Mol Cell Biol. 1993 Oct;13(10):6079-88. doi: 10.1128/mcb.13.10.6079-6088.1993. Mol Cell Biol. 1993. PMID: 8413210 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous
