Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1990 Dec;81(12):1272-80.
doi: 10.1111/j.1349-7006.1990.tb02690.x.

Identification of members of the protein phosphatase 1 gene family in the rat and enhanced expression of protein phosphatase 1 alpha gene in rat hepatocellular carcinomas

K Sasaki  1 , H Shima, Y Kitagawa, S Irino, T Sugimura, M Nagao
Affiliations
Comparative Study

Identification of members of the protein phosphatase 1 gene family in the rat and enhanced expression of protein phosphatase 1 alpha gene in rat hepatocellular carcinomas

K Sasaki et al. Jpn J Cancer Res. 1990 Dec.

Erratum in

Abstract

We isolated four kings of cDNA clones of isotypes of catalytic subunits of protein phosphatase 1 (PP-1) from rat liver and testis cDNA libraries. For the cloning, cDNA fragments of dis2ml and dis2m2, which encode mouse PP-1 catalytic subunit, were used as probes. Two of the four isotypes were thought to be derived from the same gene and produced by alternative splicing. Based on the comparative study of their nucleotide and deduced amino acid sequences with those reported, these cDNA clones were named rat PP-1 alpha, PP-1 gamma 1, PP-1 gamma 2 and PP-1 delta. The deduced amino acid sequences of these four cDNA clones showed about 90% identity. Their amino-terminal regions were highly conserved, and their differences were mainly in the carboxy-terminal regions. Furthermore, several amino acids located in the middle regions of the peptides were conserved in all the isotypes of the catalytic subunits of PP-1, PP-2A, PP-2B and PP-2C. These conserved regions are suggested to be the functional domains of the catalytic subunits of protein phosphatases. Rat hepatocellular carcinomas induced by a food mutagen, 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline showed increased expression of PP-1 alpha, but no increased expression of PP-1 gamma 1, PP-1 gamma 2 or PP-1 delta. Involvement of PP-1 alpha in hepatocarcinogenesis or in hepatic cell proliferation was suspected.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. ) Ingebritsen , T. S. and Cohen , P.The protein phosphatases involved in cellular regulation . Eur. J. Biochem. , 132 , 255 – 261 ( 1983. ). - PubMed
    1. ) Ingebritsen , T. S. and Cohen , P.Protein phosphatases: properties and role in cellular regulation . Science , 221 , 331 – 339 ( 1983. ). - PubMed
    1. ) Cohen , P.The structure and regulation of protein phosphatases . Annu. Rev. Biochem. , 58 , 453 – 508 ( 1989. ). - PubMed
    1. ) Ballou , L. M. , Brautigan , D. L. and Fischer , E. H.Subunit structure and activation of inactive phosphorylase phosphatase . Biochemistry , 22 , 3393 – 3399 ( 1983. ). - PubMed
    1. ) Hubbard , M. J. and Cohen , P.The glycogen‐binding subunit of protein phosphatase 1G from rabbit skeletal muscle: further characterisation of its structure and glycogen‐binding properties . Eur. J. Biochem. , 180 , 457 – 465 ( 1989. ). - PubMed

Publication types

LinkOut - more resources