Synthetic poliovirus and other designer viruses: what have we learned from them?
- PMID: 21756105
- DOI: 10.1146/annurev-micro-090110-102957
Synthetic poliovirus and other designer viruses: what have we learned from them?
Abstract
Owing to known genome sequences, modern strategies of DNA synthesis have made it possible to recreate in principle all known viruses independent of natural templates. We describe the first synthesis of a virus (poliovirus) in 2002 that was accomplished outside living cells. We comment on the reaction of laypeople and scientists to the work, which shaped the response to de novo syntheses of other viruses. We discuss those viruses that have been synthesized since 2002, among them viruses whose precise genome sequence had to be established by painstakingly stitching together pieces of sequence information, and viruses involved in zoonosis. Synthesizing viral genomes provides a powerful tool for studying gene function and the pathogenic potential of these organisms. It also allows modification of viral genomes to an extent hitherto unthinkable. Recoding of poliovirus and influenza virus to develop new vaccine candidates and refactoring the phage T7 DNA genome are discussed as examples.
Similar articles
-
Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template.Science. 2002 Aug 9;297(5583):1016-8. doi: 10.1126/science.1072266. Epub 2002 Jul 11. Science. 2002. PMID: 12114528
-
Virology. Active poliovirus baked from scratch.Science. 2002 Jul 12;297(5579):174-5. doi: 10.1126/science.297.5579.174b. Science. 2002. PMID: 12114601 No abstract available.
-
Production of infectious poliovirus from synthetic viral genomes.Methods Mol Biol. 2012;852:181-93. doi: 10.1007/978-1-61779-564-0_14. Methods Mol Biol. 2012. PMID: 22328434
-
Genetic stability and instability of the cis-acting control element of the 5' untranslated region of the poliovirus RNA.Dev Biol Stand. 1993;78:11-6. Dev Biol Stand. 1993. PMID: 8388821 Review.
-
Molecular biology and cell-free synthesis of poliovirus.Biologicals. 1993 Dec;21(4):349-56. doi: 10.1006/biol.1993.1095. Biologicals. 1993. PMID: 8024750 Review.
Cited by
-
Extensive recoding of dengue virus type 2 specifically reduces replication in primate cells without gain-of-function in Aedes aegypti mosquitoes.PLoS One. 2018 Sep 7;13(9):e0198303. doi: 10.1371/journal.pone.0198303. eCollection 2018. PLoS One. 2018. PMID: 30192757 Free PMC article.
-
Large-scale de novo DNA synthesis: technologies and applications.Nat Methods. 2014 May;11(5):499-507. doi: 10.1038/nmeth.2918. Nat Methods. 2014. PMID: 24781323 Free PMC article. Review.
-
Pandemic influenza A virus codon usage revisited: biases, adaptation and implications for vaccine strain development.Virol J. 2012 Nov 8;9:263. doi: 10.1186/1743-422X-9-263. Virol J. 2012. PMID: 23134595 Free PMC article.
-
Limits of variation, specific infectivity, and genome packaging of massively recoded poliovirus genomes.Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):E8731-E8740. doi: 10.1073/pnas.1714385114. Epub 2017 Sep 25. Proc Natl Acad Sci U S A. 2017. PMID: 28973853 Free PMC article.
-
Profile of Eckard Wimmer. Interview by Sujata Gupta.Proc Natl Acad Sci U S A. 2013 Feb 5;110(6):1973-5. doi: 10.1073/pnas.1221558110. Epub 2012 Dec 31. Proc Natl Acad Sci U S A. 2013. PMID: 23277540 Free PMC article. No abstract available.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
