Mechanical stress and cellular metabolism in living soft tissue composites
- PMID: 2173631
- DOI: 10.1016/0142-9612(90)90059-y
Mechanical stress and cellular metabolism in living soft tissue composites
Abstract
A soft tissue substitute composed of a three-dimensional collagen lattice infiltrated with living fibroblasts was modelled as a two-phase composite material. The phases consisted of an extracellular matrix containing fibres and fluid and cellular inclusions. A mathematical model that had been previously derived for calculating the elastic stress and energy distribution around spherical inclusions and voids in a three-dimensional elastic medium was applied to a biological composite containing cells in an elastic medium. The model predicted an increase in stress inside the cells in the direction of applied load and a decrease in the direction perpendicular to the applied load. The model also predicted increased interfacial stress at the cell-matrix boundary in the direction of applied load with maximum and minimum values at different points on the cell periphery. It was hypothesized that the interfacial stress around the cell inclusions might play a function in signal transduction. To test this hypothesis, an average unidirectional stress was applied to the boundary of a 1 cm X 2 cm X 3 mm lattice containing fibroblasts. When cells were cultured for 7 d and placed under stress of 15 g, cell growth was increased 1.7 fold, protein synthesis decreased 48% and intracellular cAMP was increased 3.7 fold in 24 h. The changes were a function of externally applied stress. Cells cultured for 14 d and placed under stress displayed similar results. The results supported the concept that soft tissue extracellular matrix depicted as an elastic medium can affect cell growth and development.(ABSTRACT TRUNCATED AT 250 WORDS)
Similar articles
-
Mechanoregulation of chondrocyte proliferation, maturation, and hypertrophy: ion-channel dependent transduction of matrix deformation signals.Exp Cell Res. 2000 May 1;256(2):383-91. doi: 10.1006/excr.2000.4847. Exp Cell Res. 2000. PMID: 10772811
-
Whole cell mechanics of contractile fibroblasts: relations between effective cellular and extracellular matrix moduli.Philos Trans A Math Phys Eng Sci. 2010 Feb 13;368(1912):635-54. doi: 10.1098/rsta.2009.0240. Philos Trans A Math Phys Eng Sci. 2010. PMID: 20047943 Free PMC article. Review.
-
Tensional homeostasis in dermal fibroblasts: mechanical responses to mechanical loading in three-dimensional substrates.J Cell Physiol. 1998 Jun;175(3):323-32. doi: 10.1002/(SICI)1097-4652(199806)175:3<323::AID-JCP10>3.0.CO;2-6. J Cell Physiol. 1998. PMID: 9572477
-
Fibroblast responses to mechanical forces.Proc Inst Mech Eng H. 1998;212(2):85-92. doi: 10.1243/0954411981533854. Proc Inst Mech Eng H. 1998. PMID: 9611999 Review.
-
Gene expression and extracellular matrix ultrastructure of a mineralizing chondrocyte cell culture system.J Cell Biol. 1991 Feb;112(3):501-13. doi: 10.1083/jcb.112.3.501. J Cell Biol. 1991. PMID: 1991793 Free PMC article.
Cited by
-
Initial Glaucoma Medication in the Hypertensive Phase Following Ahmed Valve Implantation: A Comparison of Results Achieved Using Aqueous Suppressants and Prostaglandin Analogs.J Clin Med. 2020 Feb 3;9(2):416. doi: 10.3390/jcm9020416. J Clin Med. 2020. PMID: 32028663 Free PMC article.
-
Tissue engineering science: consequences of cell traction force.Cytotechnology. 1992;10(3):225-50. doi: 10.1007/BF00146673. Cytotechnology. 1992. PMID: 1369238
-
Cell mechanics studied by a reconstituted model tissue.Biophys J. 2000 Nov;79(5):2353-68. doi: 10.1016/S0006-3495(00)76481-2. Biophys J. 2000. PMID: 11053115 Free PMC article.
-
Life-long epigenetic programming of cortical architecture by maternal 'Western' diet during pregnancy.Mol Psychiatry. 2020 Jan;25(1):22-36. doi: 10.1038/s41380-019-0580-4. Epub 2019 Nov 18. Mol Psychiatry. 2020. PMID: 31735910
-
Fibroblasts, myofibroblasts, and wound contraction.J Cell Biol. 1994 Feb;124(4):401-4. doi: 10.1083/jcb.124.4.401. J Cell Biol. 1994. PMID: 8106541 Free PMC article. Review. No abstract available.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources